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内毒素主要是G-菌细胞壁外表层结构成分,而且分子比较稳定,而生物制品如重组蛋白多由G-菌Escherichia coli.表达生产。内毒素进入人体后会引起休克、组织损伤、死亡等反应,因此控制生物制品中的内毒素十分重要。文中介绍了生物制品内毒素的祛除方法包括超滤法、离子交换法、色谱法和两相胶束系统等,并详细说明了各种方法的原理、特点、应用范围以及各种方法选择原则等。
Endotoxin is mainly G-bacteria cell wall surface structure components, and relatively stable molecules, and biological products such as recombinant protein and more by the G-bacteria Escherichia coli. Production. Endotoxin into the human body can cause shock, tissue damage, death and other reactions, so the control of endotoxin in biological products is very important. This paper introduced the biotin endotoxin removal methods including ultrafiltration, ion exchange, chromatography and two-phase micellar system, etc., and detailed description of the principles of various methods, characteristics, scope of application and various methods of selection principles .