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目的:探讨拳参水提物对正常小鼠免疫功能的影响。方法:测定胸腺、脾脏质量并计算脏器指数;碳粒廓清法测定单核巨噬细胞吞噬功能;MTT法检测T淋巴细胞增殖和NK细胞活性;鸡红细胞免疫后测定小鼠血清溶血素抗体水平;ELISA法测定血清中IL-2含量。结果:拳参水提取物能显著增强正常小鼠单核巨噬细胞的吞噬能力;上调正常小鼠免疫器官的胸腺指数和脾脏指数;促进T淋巴细胞增殖,增强NK细胞的细胞毒作用;上调血清溶血素及血清IL-2水平。结论:拳参水提取物对正常小鼠的免疫功能具有增强作用。
Objective: To investigate the effects of Quanshen water extract on the immune function of normal mice. Methods: The mass of thymus and spleen was measured and the organ index was calculated. The phagocytic function of monocyte macrophages was determined by carbon particle clearance method. The proliferation of T lymphocytes and the activity of NK cells were detected by MTT assay; The level of serum hemolysin antibody was measured after immunization with chicken red blood cells. The serum IL-2 level was determined by ELISA. Results: Quanshen water extract can significantly enhance the phagocytic ability of monocyte macrophages in normal mice; upregulate the thymus index and spleen index of immune organs in normal mice; promote the proliferation of T lymphocytes and enhance the cytotoxicity of NK cells; upregulation Serum hemolysin and serum IL-2 levels. Conclusion: Quanshen water extract can enhance the immune function of normal mice.