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目的 :探讨P5 3和nM 2 3基因表达在人非小细胞肺癌中的意义。方法 :收集本院 47例非小细胞肺癌根治标本的石蜡包埋组织。微波修复抗原、SP免疫组织化学方法染色 ,光镜观察。结果 :47例中 ,低分化癌组P5 3蛋白阳性率 88.88% ,高分化癌组P5 3蛋白阳性率 44 % ,差异有高度显著性 (P <0 .0 1)。有肺门纵隔淋巴结转移组的P5 3蛋白阳性率 82 .6 0 % ,无淋巴结转移组的P5 3蛋白阳性率 2 5 .0 0 %。差异有显著性 (P <0 .0 5 )。P5 3蛋白在肺腺癌与肺鳞癌中的表达差异无显著性 (P >0 .0 5 )。 47例中 ,肺鳞癌有肺门纵隔淋巴结转移组nM2 3阳性率2 1.42 % ;无淋巴结转移组nM 2 3阳性率 6 2 .5 0 % ,差异有高度显著性 (P <0 .0 1)。nM2 3在肺鳞癌与肺腺癌 ,在肺腺癌中有无淋巴结转移组表达及在非小细胞肺癌高、中、低 3种不同分化组表达差异均无显著性 (P >0 .0 5 )。结论 :P5 3蛋白表达程度与非小细胞肺癌的分化程度及淋巴结转移有关 ,而nM2 3能抑制肺鳞癌的转移。
Objective: To investigate the significance of P53 and nM 2 3 gene expression in human non-small cell lung cancer. Methods: Paraffin-embedded tissues of 47 patients with non-small cell lung cancer were collected. Microwave repair antigen, SP immunohistochemical staining, light microscopy. Results: The positive rate of P53 protein was 88.88% in 47 cases of poorly differentiated carcinoma and 44% of that in highly differentiated carcinoma, the difference was highly significant (P <0.01). The positive rate of P53 protein in hilar mediastinal lymph node metastasis group was 82.60%, and the positive rate of P53 protein in non-lymph node metastasis group was 25.0%. The difference was significant (P <0.05). P53 protein expression in lung adenocarcinoma and lung squamous cell carcinoma no significant difference (P> 0.05). Among the 47 cases, the positive rate of nM23 was 26.4% in lung squamous cell carcinoma and 21.4% in non-lymph node metastasis group, the difference was highly significant (P <0.01) ). The expression of nM23 in lung squamous cell carcinoma and lung adenocarcinoma, in lung adenocarcinoma with or without lymph node metastasis and non-small cell lung cancer in high, medium and low three kinds of differentiated group showed no significant difference (P> 0. 0 5). Conclusion: The expression of P53 protein is related to the differentiation degree of non-small cell lung cancer and lymph node metastasis, while nM23 can inhibit the metastasis of squamous cell lung cancer.