目的　建立一种快速而简便的次黄嘌呤 -氨基喋呤 -胸腺嘧啶 (HAT)敏感型杂交瘤细胞株的筛选方法 ,以缩短二次杂交瘤细胞制备时间。方法　在培养瓶中培养杂交瘤细胞 ,每 2～ 3天倍比提高培养液中 8-氮鸟嘌呤 (8- N)浓度 ,从 1.2 5μg/ ml直至 2 0 μg/ ml,并用酶联免疫吸附试验 (EL ISA)比较筛选前后抗体吸光度 (A)值。结果　成功地诱导筛选后 ,获得HAT敏感的次黄嘌呤 -鸟嘌呤磷酸核糖转移酶 (HGPRT)缺陷型杂交瘤细胞 2株 ,均保留分泌单抗功能 ,其单抗 A值与 8- N筛选培养前无明显差异。结论　改进后的筛选方法较常规方法所需时间缩短 1.0～ 2 .5倍 Objective To establish a rapid and simple screening method for hypoxanthine-aminopterin-thymidine (HAT)-sensitive hybridoma cell lines to shorten the preparation time of secondary hybridoma cells. Methods Hybridoma cells were cultured in culture flasks and the concentration of 8-azaguanine (8-N) in the culture fluid was increased every 2 to 3 days, from 1.2 5 μg/ml up to 20 μg/ml, and enzyme-linked immunosorbent assay was used. The test (EL ISA) compares the absorbance (A) values ​​of the antibodies before and after screening. Results Two strains of HAT-sensitive hypoxanthine-guanine phosphoribosyl transferase (HGPRT)-defective hybridoma cells were obtained after successful induction of selection. Both of them retained the function of monoclonal antibody, and the monoclonal antibody A value and 8-N screening culture. No significant difference before. Conclusion The improved screening method is 1.0 to 2.5 times shorter than the conventional method.