论文部分内容阅读
蛋白质缺乏对T细胞依赖性免疫的影响,目前尚无定论,有的认为T细胞功能受损,也有的是相反的意见,甚至认为有促进作用。本文作者通过小鼠实验对此问題进行了研究。选用雌性BDF_1(C 57 BL/6×DBA/2)小鼠,生后第3周断奶,分别以含4%及24%蛋白质的饲料喂养。3周后,用100μg卵白蛋白(OVA)及5μl弗氏完全佐剂足垫皮内注射,使之致敏。于免疫后第3周及第11周眶静脉取血,用ELISA方法测血清中抗OVA抗体的含量。
The effect of protein deficiency on T cell-dependent immunity is not conclusive at present, and some people think that T cell function is impaired, while others are contradictory opinions and even think it has a promoting effect. The author of this study by mouse experiments. Female BDF_1 (C57BL / 6 × DBA / 2) mice were weaned at the third week after birth and fed with 4% and 24% protein respectively. Three weeks later, sensitization was induced by intradermal injection of 100 μg ovalbumin (OVA) and 5 μl Freund’s complete adjuvant footpad. At 3 weeks and 11 weeks after immunization, orbital venous blood was collected, and the content of anti-OVA antibody in serum was measured by ELISA.