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本研究观察了的Pb~(2+)对大鼠肾微粒体膜物理状态的影响。结果表明,Pb~(2+)能明显增加ANS与膜结合后的荧光强度和荧光偏振度,但不改变荧光光谱特性。用ANS荧光滴定法研究发现,Pb~(2+)能降低膜ANS复合物的表观解离常数和增加膜上ANS的结合位点数。提示,Pb~(2+)能降低离体大鼠肾微粒体膜流动性和膜表面负电荷密度。
This study investigated the effect of Pb 2+ on the physical status of rat renal microsomes. The results show that Pb 2+ can significantly increase the fluorescence intensity and fluorescence polarization degree of ANS bound to the membrane, but does not change the fluorescence spectral characteristics. ANS fluorescence titration study found that Pb ~ (2+) can reduce the membrane ANS complex apparent dissociation constant and increase the membrane ANS binding sites. Tip, Pb ~ (2+) can reduce the in vitro rat renal microsomal membrane fluidity and membrane surface negative charge density.