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用肝穿刺采取５２例不同病日的各临床类型的肾综合征出血热（ＨＦＲＳ）患者肝组织，光镜下观察肝细胞普遍肿胀、变性，伴有灶性及大片状坏死，并可见微循环障碍。电镜观察到肝细胞浆内、肿胀的粗面内质网空泡内、内皮细胞内及微绒毛处有汉坦病毒颗粒及包涵体。３～１２病日的１９份肝活检组织培养至第３代，其中５例直接荧光和间接荧光检测均呈阳性，其中１株继续传代，繁殖良好，定名为Ｌ２０株。为了排除体内免疫因素的干扰，将Ｌ２０株及四型国际标准株感染体外培养的单层肝细胞，结果９株病毒均能在其中高滴度地繁殖。对Ｌ２０株生物学特性进一步研究，发现Ｌ２０株较国内报告的汉坦病毒的抗原性和基因特征有一定特殊性。研究结果说明汉坦病毒可直接造成肝损害。
Liver samples of 52 patients with various clinical types of hemorrhagic fever with renal syndrome (HFRS) on different days were collected by liver biopsy. The hepatocytes were generally swollen, degenerated, accompanied with focal and large necrosis by light microscopy. Circulatory disorders. Electron microscopy showed hepatocyte cytoplasm, swollen rough endoplasmic reticulum vacuoles, endothelial cells and microvilli at Hantavirus particles and inclusion bodies. Nineteen liver biopsy specimens from 3 to 12 days were cultured to the third generation, of which 5 were positive by both direct and indirect fluorescence detection. One of them continued to passage and reproduction well and was named as L20 strain. In order to rule out the interference of the immune factors in vivo, L20 strains and four types of international standard strains were infected with monolayer hepatocytes cultured in vitro. As a result, all nine viruses could multiply in high titer. Further studies on the biological characteristics of L20 strain revealed that the L20 strain has a certain degree of specificity compared with the reported Hantavirus antigenicity and gene characteristics in China. The results suggest that Hantaan virus can directly cause liver damage.