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目的 :对临床诊断严重急性呼吸道综合征 (SevereAcuteRespiratorySyndrome,SARS ,即非典型肺炎 )患者血抗SARS冠状病毒IgG抗体的变化规律和密切接触SARS患者的医护人员有无隐性感染作初步调查。 方法 :用酶联免疫吸附测定 (ELISA)检测 5 7例正常人、12 7例在SARS病房工作 1个月的医护人员和 73例不同病程SARS患者血浆抗SARS冠状病毒IgG抗体的水平。 结果 :正常人和医护人员血浆中尚未检测到抗SARS冠状病毒IgG抗体。SARS患者发病第 0～ 7、8～ 10、11～ 14、15～ 2 0天后血浆中抗SARS冠状病毒IgG抗体的阳性率分别为 0、33%、5 2 %和 86 % ,总阳性率为 6 1%。结论 :ELISA检测血浆中抗SARS冠状病毒IgG抗体的特异性和敏感度均很好。呈阳性反应病例可确诊已受病毒感染 ,对于发病早期还未产生抗体的病人 ,阴性结果不能说明未受感染 ,应做进一步观察。本组资料提示SARS可能不具有隐性感染性
Objective: To investigate the changes of blood anti-SARS coronavirus IgG antibodies in patients with severe acute respiratory syndrome (SARS) and the presence or absence of latent infection in health-care workers in close contact with SARS. Methods: The levels of anti-SARS coronavirus (IgG) antibodies against SARS coronavirus in 57 normal controls, 127 medical staff working in SARS wards and 73 SARS patients with different course of disease were detected by enzyme linked immunosorbent assay (ELISA). Results: No anti-SARS coronavirus IgG was detected in plasma of normal people and medical staff. The positive rates of anti-SARS coronavirus IgG in plasma from 0 to 7, 8 to 10, 11 to 14, and 15 to 20 days after onset of SARS were 0, 33%, 52% and 86%, respectively. The overall positive rates were 6 1%. Conclusion: The specificity and sensitivity of ELISA for the detection of anti-SARS coronavirus IgG in plasma are good. Positive cases can be diagnosed infected with the virus, for the early onset of antibodies have not yet produced patients, the negative results can not explain the uninfected, should be further observed. The data suggest that SARS may not be recessive infectivity