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通过软琼脂克隆法及有限稀释法,从(Hca-F25/L)细胞系中分离出五株不同转移力的克隆细胞,它们的共同特点是:均沿淋巴系统转移,仅有极少数(1.88%)同时伴有肺转移;五株克隆及其亲代对角蛋白单抗(HK2)均呈现阳性,提示尽管它们分化很低,但仍保持了上皮组织的特性;经染色体分析证实,每一克隆内同一核型的细胞占绝大多数,它们和亲代有相同的四个标记染色体,表明它们之间有“血缘”关系。它们的不同之处表现在:在615小鼠体内均能致瘤(100%),但在淋巴结中的转移率及其转移程度有很大的差别,其中一株高转移细胞系(78.6%—89.4%),三株中转移细胞系(33.3%—66.6%),一株低转移细胞系(15%～15.4%);五株克隆及其亲代的干系核型也各不相同;在细胞形态上和细胞电泳率方面,高、低转移细胞系与亲代细胞系三者之间有明显的差别。本文较确切地证明了 Hca 瘤株在转移表型和核型上的异质性。同时,也补充和发展了 H22/Hca 肿瘤系统。
Using the soft agar cloning method and the limiting dilution method, five strains of cells with different transfer forces were isolated from the (Hca-F25/L) cell line. Their common feature was that they all migrated along the lymphatic system with only a few (1.88). (%) was accompanied by lung metastases; five clones and their parents were positive for keratin monoclonal antibody (HK2), suggesting that despite their very low differentiation, they retained the characteristics of epithelial tissues; chromosomal analysis confirmed each clone. The cells that share the same karyotype account for the vast majority. They have the same four marker chromosomes as the parent, indicating a “blood” relationship between them. Their differences are as follows: in 615 mice can be tumorigenic (100%), but the lymph node metastasis rate and its degree of transfer are very different, in which a highly metastatic cell line (78.6% - 89.4%), three metastatic cell lines (33.3%-66.6%), one low-metastatic cell line (15%-15.4%); five clones and their parental karyotypes are also different; in cell morphology On the electrophoretic rate of cells and cells, there were significant differences between the high and low metastatic cell lines and the parental cell lines. This article more accurately demonstrated the heterogeneity of the Hca tumor line on metastatic phenotype and karyotype. At the same time, the H22/Hca tumor system was also supplemented and developed.