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以成年老年人股骨颈部松质骨为材料，采用酶法建立人成骨细胞体外模型，结果示：（１）来源于松质骨的成骨细胞早期以多角形、梭形为主，后期以梭形为主；（２）成骨细胞早期有黑色颗粒分泌，２ 周后出现黑色结节；（３）骨钙素的分泌以第１４ 天左右达到最高，以后则递减。本法有取材方便，可得到大量、纯化的成骨细胞的优点。
The cancellous bone in the femoral neck of adult and old people was used as material to establish human osteoblast model in vitro. The results showed that: (1) osteoblasts derived from cancellous bone were mainly polygons and fusiform in the early stage, Mainly spindle; (2) the early osteoblast excretion of black particles, 2 weeks after the emergence of black nodules; (3) osteocalcin secretion to reach the highest on the 14th day, then decreased. This method has access to convenient, can be a large number of purified osteoblasts advantages.