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研究大鼠在戊四氮导致癫痫发作早期前脑内小胶质细胞的变化及其与神经元的关系,本研究应用免疫组织化学法分别显示前脑内OX-42和Fos蛋白表达的时程变化,并用双重标记显示OX-42和Fos阳性细胞的相互关系。结果发现:在戊四氮导致大鼠癫痫发作早期(从15min到360min),前脑的小胶质细胞OX-42表达阳性,随着存活时间的变化,OX-42的阳性反应经历逐渐升高又降低的过程;Fos蛋白在神经元和小胶质细胞中有表达,也呈现逐渐升高又降低的变化;Fos在小胶质细胞表达高峰的时间早于在神经元的表达;另外OX-42阳性小胶质细胞和Fos阳性神经元在前脑分布基本相同,主要分布在大脑皮层、海马、杏仁核等部位。以上结果表明,前脑的小胶质细胞和神经元一样在戊四氮所致癫痫发作的早期表现明显的反应,但小胶质细胞反应的意义有待进一步研究。
To investigate the changes of microglia in the forebrain and the relationship with neurons in the early stage of pentylenetetrazole-induced seizures in rats, we used immunohistochemistry to show the time course of OX-42 and Fos expression in the forebrain Changes and double-labeled to show the correlation between OX-42 and Fos-positive cells. The results showed that the positive expression of OX-42 in the microglia of the forebrain was positive in the early stage of pentylenetetrazole-induced seizures (from 15min to 360min), and the positive reaction of OX-42 gradually increased with the survival time Fos protein expression in neurons and microglia also showed gradually increased and decreased changes; Fos expression peak in microglia earlier than in neurons; the other OX- 42 positive microglial cells and Fos positive neurons in the forebrain are basically the same, mainly in the cerebral cortex, hippocampus, amygdala and other parts. The above results indicate that microglial cells and neurons in the forebrain exhibit obvious responses in the early stage of seizures caused by pentylenetetrazole, but the significance of microglia reaction remains to be further studied.