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目的建立能同时检测肉类食品中20种磺胺和8种喹诺酮类抗生素残留的Qu ECh ERS-超高效液相色谱-串联质谱法(UPLC-MS/MS)。方法样品加1%甲酸乙腈提取,经分散固相萃取净化后,采用Waters C18(2.1 mm×100 mm,1.7μm)色谱柱分离,梯度洗脱,采用电喷雾-正离子多反应监测模式,外标法定量。结果 28种抗生素在各自浓度范围内线性关系良好,相关系数(r)为0.997 5~0.999 6,检出限(LOD)为0.17~0.91μg/kg(S/N=3),磺胺类抗生素回收率在70.9%~115.2%之间,RSD在2.8%~15.9%(n=6)之间;喹诺酮类抗生素回收率在71.3%~112.3%之间,RSD在2.5%~14.6%(n=6)之间。结论建立的方法准确、快速、灵敏度高,可对动物源性食品中多种抗生素残留进行快速分析。
Objective To establish a Qu ECh ERS-UPLC-MS / MS method capable of simultaneously detecting 20 sulfonamides and 8 quinolone antibiotics in meat products. Methods The sample was extracted with 1% formic acid and extracted with acetonitrile. The sample was purified by dispersive solid-phase extraction. The sample was separated on a Waters C18 (2.1 mm × 100 mm, 1.7 μm) column using gradient elution. The electrospray ionization- Standard method of quantitative. Results The results showed that the correlation coefficient (r) was 0.997 5 to 0.999 6 and the limit of detection (LOD) was 0.17 to 0.91 μg / kg (S / N = 3) for all 28 antibiotics. The recovery of sulfonamides The RSD ranged from 2.8% to 15.9% (n = 6). The recoveries of quinolone antibiotics ranged from 71.3% to 112.3% with RSD ranging from 2.5% to 14.6% (n = 6 )between. Conclusion The established method is accurate, rapid and sensitive. It can rapidly analyze many kinds of antibiotic residues in animal-derived foods.