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笔者建立了来源于人脑颞枕部和顶部的两个胶质母细胞瘤的体外细胞系,目前已分别存活5年,传500余代。 方法与结果 一、细胞系的建立:1.材料来源:TJ899细胞系取材于1例男性在颞枕部胶质瘤患者的手术切除标本。TJ905体外细胞系取材于1例男性顶部胶质瘤患者。二者均经病理组织学诊断为多形性胶质母细胞瘤。2.建系经过:在无菌条件下,用Hanks液漂洗瘤组织,除去血液及肉眼可见的电灼及坏死组织。机械离碎,将组织块接种于25cm~2培养瓶内,加入含15％胎牛血清(FCS)的Way Mouth MAB 87/3(Gibco Lab,USA)培养液,37℃、5％CO_2孵育72小时。大多数组织块均有不同程度细胞移出,胞体较小,1周后多数培养瓶壁已长满细胞。经0.125％胰蛋白酶
The author established an in vitro cell line derived from two glioblastomas at the temporal occipital and top of the human brain and has now survived for 5 years respectively and passed over 500 generations. Methods and Results 1. Establishment of cell lines: 1. Source of material: TJ899 cell line is based on a surgical resection of 1 male with temporal occipital glioma. TJ905 in vitro cell lines derived from 1 case of male patients with the top glioma. Both were diagnosed by histopathology as glioblastoma multiforme. 2. After the establishment of Department: In aseptic conditions, rinse the tumor tissue with Hanks solution to remove the blood and visible necrotic and necrotic tissue. The tissue pieces were inoculated into 25cm 2 flasks and cultured in Way Mouth MAB 87/3 (Gibco Lab, USA) supplemented with 15% fetal bovine serum (FCS) at 37 ° C in 5% CO 2 for 72 hour. Most tissue blocks have different degrees of cell removal, cell body is small, most of the culture bottle wall has been covered with cells after 1 week. After 0.125% trypsin