目的探讨PLK1基因在宫颈组织中的表达情况及其与临床病理特征之间的关系。方法采用实时荧光定量RT-PCR法检测各组m RNA的表达量。计算各组表达量采用相对定量法,检测的组织中正常宫颈组(10例),宫颈上皮内瘤变组(12例),癌症组(26例)。结果随着癌的进展,各组表达量具有上升趋势,运用统计学软件和方法比较差异性表达是否有意义,结果显示,宫颈上皮内瘤变组与正常组统计学比较有意义(P<0.05),癌症组与宫颈上皮内瘤变组和正常组比较均具有统计学意义(P<0.05)。分析癌症组与某些临床特征之间的关系显示,PLK1与肿瘤细胞分化程度有关(P<0.05),与患者年龄、临床分期无关(P>0.05)。结论 PLK1与宫颈癌的发生有一定的作用,有望成为协助诊断宫颈癌的一个预测指标,并为癌的靶基因治疗提供了帮助。 Objective To investigate the expression of PLK1 in cervical tissue and its relationship with clinicopathological features. Methods The expression of m RNA in each group was detected by real-time fluorescent quantitative RT-PCR. The expression of each group was calculated by relative quantification method, the normal cervical tissue (10 cases), cervical intraepithelial neoplasia group (12 cases) and cancer group (26 cases) were detected. Results With the progress of cancer, the expression of each group had an upward trend. The statistical software and methods were used to compare whether the differential expression was significant. The results showed that there was statistical significance between the cervical intraepithelial neoplasia group and the normal group (P <0.05 ), Cancer group and cervical intraepithelial neoplasia group and normal group were statistically significant (P <0.05). Analysis of the relationship between cancer group and some clinical features showed that PLK1 was related to tumor cell differentiation (P <0.05), but not related to patient’s age and clinical stage (P> 0.05). Conclusions PLK1 plays a role in the carcinogenesis of cervical cancer and is expected to become a predictor of cervical cancer and help to target the gene therapy of cancer.