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目的探讨不同补肾复方补益肾气为主之毓麟珠、滋养肾阴为重之养精种玉汤、温补肾阳为要之温胞饮在体外受精-胚胎移植过程中对小鼠卵细胞质量的影响。方法将KM小鼠随机分为5组,对照组和激素组给予蒸馏水灌胃10 g·kg-1,毓麟珠组、养精种玉汤组和温胞饮组分别给予毓麟珠浸膏相当于生药16 g·kg-1、养精种玉汤浸膏相当于生药10 g·kg-1、温胞饮浸膏相当于生药13 g·kg-1,每日1次,连续15 d。最后1次给药后,激素组腹腔注射PMSG 10U/只,中药各组、对照组注射等量生理盐水。48h后腹腔注射HCG 10U/只。观察小鼠排卵情况及卵细胞形态和体外受精及卵裂情况。结果激素组可增加小鼠排卵数/个(34.30±11.64),各中药组较对照组、激素组卵细胞正常率高/个(%)[89(76.07),80(76.19),83(75.45%)vs 48(57.83),188(54.81)],P<0.05或P<0.01;各中药组较激素组受精率/个(%)[47(40.17%),42(40.00),43(39.09)vs 71(20.70)]、卵裂率/个(%)[22(46.81),19(45.24),19(44.19)vs 19(26.76)]、卵裂球正常率/个(%)[19(86.36),16(84.21),16(84.21)vs 13(68.42)]均高(P<0.05或P<0.01)。结论不同补肾复方补益肾气为主之毓麟珠、滋养肾阴为重之养精种玉汤、温补肾阳为要之温胞饮均可促进小鼠卵细胞成熟,并能改善卵细胞质量。毓麟珠作用尤为显著。
Objective To investigate the effects of different kidney-tonifying kidney-qi supplements on Yulin beads, nourishing the kidney and yin-nourishing Jingjing jade soup, and warming yin and kidney yang in the process of in vitro fertilization and embryo transfer on the quality of mouse egg influences. Methods KM mice were randomly divided into five groups, the control group and the hormone group were given distilled water gavage 10 g · kg-1, Yulin beads group, Yang Jing Jing Yu Decoction group and Wen Zhu Yin group were given Yulin beads extract Which is equivalent to 16 g · kg-1 of crude drug, Yangjing Decoction is equivalent to 10 g · kg-1 of crude drug, and Wenzhu Decoction is equivalent to 13 g · kg-1 of crude drug once daily for 15 days . After the last administration, the hormone group was intraperitoneally injected with PMSG 10U / kg, the Chinese medicine group, the control group injected with the same amount of saline. 48h after intraperitoneal injection of HCG 10U / only. Ovulation was observed in mice and egg morphology and in vitro fertilization and cleavage. Results The hormones increased the number of ovulation per mouse (34.30 ± 11.64), and the normal rates of oocyte in control group and hormone group were 89% (76.07), 80 (76.19%) and 83% (75.45% ) vs 48 (57.83), 188 (54.81)], P <0.05 or P <0.01. Compared with the hormone group, the fertilization rate of each TCM group was 47% (40.17%), 42% (40.00%), 43% vs 71 (20.70)], cleavage rate / month (%) [22 (46.81), 19 (45.24), 19 (44.19) vs 19 86.36), 16 (84.21), 16 (84.21) vs 13 (68.42)] (all P <0.05 or P <0.01). Conclusion Different kidney nourishing kidney-qi, kidney-yin, nourishing yin-yin, nourishing kidney and yin, nourishing kidney and yang for warming can promote mouse egg cell maturation and improve egg mass. Yulin beads effect is particularly significant.