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为了明确旱稻×稗草杂交后代YF2-1对渗透胁迫的耐性是否得到了改善,以YF2-1及其母本旱稻品种H65为材料,在苗期采用PEG-6000进行渗透胁迫处理,研究它们的气体交换参数、叶绿素荧光参数及抗氧化酶活性对渗透胁迫的响应。结果表明,在渗透胁迫处理下,YF2-1更能维持较高的净光合速率(Pn)和气孔导度(Gs)。这可能与YF2-1具有更好的持水能力,并能有效地通过热耗散(NPQ高)消耗过剩光能,通过高活性的抗氧化酶诱导来清除活性氧,从而避免活性氧伤害相关。旱稻与稗草的远源杂交提高了旱稻对渗透胁迫的抗性。
In order to clarify whether YF2-1 tolerance to osmotic stress was improved in hybrids of upland rice and barnyardgrass, we used PEG-6000 to treat osmotic stress (YF2-1) and its parent upland rice (H65) Gas exchange parameters, chlorophyll fluorescence parameters and antioxidant enzyme activities under osmotic stress. The results showed that under osmotic stress, YF2-1 could maintain higher net photosynthetic rate (Pn) and stomatal conductance (Gs). This may have better water-holding capacity than YF2-1 and effectively dissipate excess light energy through heat dissipation (NPQ high), reactive oxygen species elimination by highly active antioxidant enzymes and thus avoid reactive oxygen species damage . Distal hybridization of upland rice and barnyardgrass increased the resistance of upland rice to osmotic stress.