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目的:观察和研究异常黑胆质型肝癌病证模型免疫器官的组织形态及其免疫功能的变化,进一步阐明该病证模型的免疫学本质。方法:在异常黑胆质证载体动物模型基础上用二乙基亚硝胺(DEN)诱导建立异常黑胆质型肝癌病证模型,分别采用光学显微镜和透射电镜观察其免疫器官(脾脏和胸腺)组织病理和细胞超微结构的变化,并检测外周血白介素-1β(IL-1β)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)。结果:与正常对照组比较,对照肝癌组、异常黑胆质证组和病证模型组的免疫器官存在组织形态学和超微结构的改变;且与对照肝癌组比较,病证模型组损伤性病理改变更加显著;与正常对照组比较,病证模型组外周血清IL-1β、IL-6和TNF-α水平显著升高(P<0.01);与对照肝癌组比较,病证模型组IL-6和TNF-α水平显著升高(P<0.01)。结论:异常黑胆质型肝癌病证模型不仅存在免疫器官组织形态的病理性改变,而且具有细胞免疫功能的严重失衡,并且这些结构及功能上的病理性改变较模型对照组为甚,提示异常黑胆质证可能以其强刺激性的特点,引发机体免疫器官组织形态病理性变化,破坏机体细胞免疫功能的平衡,从而促进和加速异常黑胆质型肝癌病证模型发生和发展的过程。
OBJECTIVE: To observe and study the histological changes and immune function of immune organs in the abnormal savda hepatocarcinoma model and to further elucidate the immunological essence of this syndrome model. Methods: Abnormal savda hepatocarcinoma model was induced by diethylnitrosamine (DEN) on the basis of the animal model of abnormal savda. The immune organs (spleen and thymus) were observed by optical microscope and transmission electron microscope respectively. The changes of histopathology and ultrastructure were observed. The levels of interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF- α) were detected. Results: Compared with the normal control group, the histopathological and ultrastructural changes of the immune organs in the control hepatoma group, the abnormal savda syndrome group and the disease-syndrome model group were observed. Compared with the control group, the lesion pathological changes Compared with the control group, the levels of IL-1β, IL-6 and TNF-α in the peripheral blood of the model group were significantly increased (P <0.01). Compared with the control group, the levels of IL-6 And TNF-α levels were significantly increased (P <0.01). Conclusion: The model of abnormal savda hepatocarcinoma not only presents pathological changes of the morphology of immune organs, but also has a serious imbalance of cellular immunity, and these structural and functional pathological changes are more serious than the model control group, suggesting abnormalities The savda syndrome may trigger the histopathological changes of the immune organ tissue and the balance of cell immune function by its strong irritant characteristics so as to promote and accelerate the occurrence and development of the abnormal savda hepatoblasts syndrome model.