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目的 利用树突状细胞呈递肿瘤抗原的特性提高细胞毒T淋巴细胞 (CTLs)对胃癌细胞的杀伤活性。方法 胃细胞癌患者外周血来源的有核细胞体外经GM -CSF和IL -4诱导产生树突状细胞 ,负载肿瘤裂解物后诱导自体CTLs产生。用细胞毒试验检测CTLs杀伤活性 ,和用ELISA测定细胞因子的分泌。结果 胃癌患者自体来源的DC裂解物能诱导产生的CTLs对自体胃癌细胞具有高杀伤率 ,可达 83 % ;致敏的DC组中IL -12与TNF -α的浓度 ( 1161± 2 3 9pg/ml,10 44± 3 12 pg/ml)显著高于未致敏的DC组 ( P <0 .0 5 )。结论 DC能呈递胃癌裂解物 ,诱导产生抗原特异性CTLs。
Objective To improve the cytotoxic activity of cytotoxic T lymphocytes (CTLs) on gastric cancer cells by using the characteristics of dendritic cells presenting tumor antigens. Methods Peripheral blood mononuclear cells from patients with gastric cancer were induced by GM-CSF and IL-4 to produce dendritic cells, which were loaded with tumor lysate and induced to produce autologous CTLs. CTLs killing activity was tested by cytotoxicity assay and cytokine secretion was measured by ELISA. Results CTLs induced by autologous DC lysates of gastric cancer patients had a high killing rate of autologous gastric cancer cells up to 83%. The concentrations of IL-12 and TNF-α in sensitized DCs group (1161 ± 239pg / ml, 10 44 ± 3 12 pg / ml) was significantly higher than the non-sensitized DC group (P <0.05). Conclusion DC can present gastric cancer lysate and induce the production of antigen-specific CTLs.