论文部分内容阅读
RGA(抗性基因同源序列)法是克隆植物抗性基因的一种经济有效的方法,成为近年来的研究热点。本实验综合分析了拟南芥,西红柿,水稻,烟草等植物已克隆的抗性基因,并以这些抗性基因的NBS(核酸结合位点),LRR(富含亮氨酸重复),STK(丝氨酸/苏氨酸激酶)保守结构域设计并合成了几十对RGA引物,对小麦抗条锈病材料进行PCR扩增,获得以Xal-NBS为引物的R88RGA片段,经克隆和序列比对分析,发现该片段与逆境条件下植物抗病信号传导相关,与蛋白激酶同源性达到96%。此项研究对抗病机理的研究和基因的发掘有重要的指导意义。
RGA (resistance gene homologous sequence) method is a cost-effective method for cloning plant resistance genes and has become a research hotspot in recent years. In this study, the cloned resistance genes of Arabidopsis, tomato, rice, tobacco and other plants were comprehensively analyzed. The resistance genes such as NBS (Nucleotide Binding Site), LRR (Leucine Rich Repeat), STK Serine / threonine kinase) conserved domain design and synthesis of dozens of pairs of RGA primers for wheat stripe rust resistance of the material was amplified to obtain Xal-NBS as a primer R88RGA fragment by cloning and sequence alignment analysis, The fragment was found to be associated with plant resistance to signal transduction under stress conditions and had 96% homology to protein kinase. This research has important guiding significance on the research of disease resistance mechanism and gene discovery.