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目的:探讨长链非编码RNA（lncRNA）CTA-796E4.4在膀胱癌组织和细胞株中的表达，并观察其对膀胱癌细胞增殖和侵袭能力的影响及其分子机制。方法:采用荧光实时定量聚合酶链反应（qPCR）法检测湖北省天门市第一人民医院2016年11月至2019年1月手术切除标本73例膀胱癌组织及癌旁组织、4种膀胱癌细胞株（UM-UC-3、BIU-87、5637、T24）及正常膀胱上皮细胞中CTA-796E4.4的表达。以携带CTA-796E4.4基因的重组慢病毒（LV-CTA-796E4.4）感染UM-UC-3细胞作为实验组，以阴性对照慢病毒（LV-NC）感染UM-UC-3细胞为对照组。采用qPCR法检测过表达CTA-796E4.4对叉头框转录因子O1（FOXO1）基因mRNA表达的影响，通过Western blotting检测FOXO1蛋白的表达，分别以噻唑蓝（MTT）法、Transwell侵袭实验检测高表达CTA-796E4.4对UM-UC-3细胞增殖和侵袭能力的影响。结果:CTA-796E4.4在膀胱癌组织中的表达水平低于癌旁组织（1.22 ± 0.33比4.30 ± 0.64），差异有统计学意义（n P<0.01）。CTA-796E4.4在膀胱癌细胞株（UM-UC-3、BIU-87、5637、T24）中的表达均低于膀胱上皮细胞（0.11 ± 0.03、0.61 ± 0.03、0.33 ± 0.03、0.73 ± 0.04比1.01 ± 0.10）（n P<0.01）。LV-CTA-796E4.4感染细胞后，CTA-796E4.4表达量显著增加（n P<0.01），FOXO1基因表达升高（n P<0.01）。高表达CTA-796E4.4能明显抑制细胞增殖能力和侵袭能力（n P<0.05）。n 结论:lncRNA CTA-796E4.4在膀胱癌及细胞株中表达降低，高表达CTA-796E4.4可抑制UM-UC-3细胞的增殖和侵袭，其分子机制可能是上调CTA-796E4.4促进FOXO1基因的表达。“,”Objective:To investigate the expression of long-chain noncoding RNA (lncRNA) CTA-796E4.4 in bladder cancer tissues and cell lines, and to observe its effect on the proliferation and invasion of bladder cancer cells and to explore its possible molecular mechanism.Methods:The expression of CTA-796E4.4 in 73 bladder cancer tissues and adjacent tissues, 4 kinds of bladder cancer cell lines (UM-UC-3, BIU-87, 5637, T24) and normal bladder epithelial cells in the First People′s Hospital of Tianmen City from November 2016 to January 2019 were detected by qPCR. UM-UC-3 cells infected with recombinant lentivirus (LV-CTA-796E4.4) carrying the CTA-796E4.4 gene were taken as an experimental group, and UM-UC-3 cells infected with negative control lentivirus (LV-NC) were as a control group. The effect of over expression of CTA-796E4.4 on the expression of Forkhead box transcription factor O1 (FOXO1) mRNA was detected by qPCR. The expression of FOXO1 protein was detected by Western blotting, Thiazolyl blue (MTT) method Transwell invasion assay was respectively used to examine the effect of high expression of CTA-796E4.4 on proliferation and invasion of UM-UC-3 cells.Results:The expression level of CTA-796E4.4 in bladder cancer tissue was lower than that in para cancerous tissue (1.22 ± 0.33 vs. 4.30 ± 0.64) and the difference was statistically significant (n P < 0.01). The expression of CTA-796E4.4 in bladder cancer cells was lower than that in bladder cancer epithelial cells (0.11 ± 0.03, 0.61 ± 0.03, 0.33 ± 0.03, 0.73 ± 0.04 vs.1.01 ± 0.10) ( n P < 0.01). After LV-CTA-796E4.4 infection, the expression of CTA-796E4.4 was significantly increased ( n P < 0.01) and the expression of FOXO1 gene was increased ( n P < 0.01). High expression of CTA-796E4.4 significantly inhibited cell proliferation and invasion of UM-UC-3 cells ( n P < 0.05).n Conclusions:The expression of lncRNA CTA-796E4.4 is decreased in bladder cancer and cell lines. High expression of CTA-796E4.4 inhibites the proliferation and invasion of UM-UC-3 cells. The molecular mechanism may be that up-regulation of CTA-796E4.4 can promote the expression of FOXO1.