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分析不同环境压力条件(温度、pH、渗透压和超高压)对12株致病性副溶血性弧菌耐药性的影响。利用微量肉汤稀释法测定菌株在不同温度(37℃和30℃)、渗透压(1%和6%NaCl)、pH(6.0和9.0)及超高压(180、250和300 MPa)条件影响下,其对所测抗生素的最小抑菌浓度(MIC)。结果表明,在1%和6%NaCl质量分数、pH 9.0条件下,副溶血性弧菌的耐药性增强,而在30℃、pH 6和超高压条件影响下,其耐药性减弱。此外,菌株在所测环境压力条件影响下,其对环丙沙星和头孢类抗生素的耐药性基本保持不变。环境压力条件的改变可能会增强致病性副溶血性弧菌的耐药性。
The effects of different environmental pressure conditions (temperature, pH, osmotic pressure and ultrahigh pressure) on the pathogenicity of 12 pathogenic Vibrio parahaemolyticus were analyzed. The effects of different temperature (37 ℃ and 30 ℃), osmotic pressure (1% and 6% NaCl), pH (6.0 and 9.0) and ultra-high pressure (180, 250 and 300 MPa) , Its minimum inhibitory concentration (MIC) on the antibiotic tested. The results showed that the drug resistance of Vibrio parahaemolyticus was enhanced under the conditions of 1% and 6% NaCl, pH 9.0, and the drug resistance of Vibrio parahaemolyticus was weakened under the conditions of 30 ℃, pH 6 and ultrahigh pressure. In addition, the strain under the influence of environmental pressure conditions, its ciprofloxacin and cephalosporins antibiotic resistance remained unchanged. Changes in environmental stress conditions may enhance the resistance of pathogenic Vibrio parahaemolyticus.