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目的:探讨白介素-1受体相关激酶(IRAK-4)在重症腹内感染大鼠肝损伤的作用机制,以及通里攻下方剂的治疗作用。方法:通过建立大鼠重症腹内感染模型,并随机分为假手术组、模型组和中药治疗组。每组分3、6、12、24h 4个时间亚组,检测每组肝脏组织IRAK-4蛋白表达水平、门静脉内毒素水平及血浆肿瘤坏死因子-α(TNF-α)含量,观察肝组织HE染色结果。结果:模型组和中药治疗组在造模后各个时间点IRAK-4蛋白表达水平较假手术组均明显升高(P<0.05);中药治疗组与模型组比较,在24h时显著降低(P<0.05);模型组肝脏IRAK-4蛋白表达水平与门静脉内毒素变化、血浆TNF-α含量呈正相关。结论:IRAK-4蛋白表达水平升高可能是内毒素信号传导通路导致肝脏损伤的重要因素;通里攻下方剂可抑制IRAK-4激酶活性,减轻重症腹内感染大鼠肝脏损伤的程度。
Objective: To investigate the mechanism of interleukin-1 receptor-related kinase (IRAK-4) in liver injury induced by severe intra-abdominal infection in rats and the therapeutic effect of the prescription of Tongkailiao. Methods: The model of severe intra-abdominal infection in rats was established and randomly divided into sham operation group, model group and Chinese medicine treatment group. Each group 3,6,12,24 h 4 time subgroups, each group of liver tissue IRAK-4 protein expression, endotoxin levels and plasma levels of tumor necrosis factor-α (TNF-α) were observed liver tissue HE Dyeing results. Results: The expression of IRAK-4 in model group and traditional Chinese medicine group were significantly higher than those in sham group (P <0.05) at each time point after model establishment. Compared with model group, the expression of IRAK- <0.05). The expression of IRAK-4 protein in liver of model group was positively correlated with the changes of portal venous endotoxin and plasma TNF-α. CONCLUSION: Elevated IRAK-4 protein expression may be an important factor in hepatic injury induced by endotoxin signal transduction pathway. It may inhibit the activity of IRAK-4 kinase and decrease the severity of hepatic injury in rats with severe intra-abdominal infection.