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目的建立鸡肉中金刚烷胺的液相色谱-串联质谱测定方法。方法鸡肉样品中的金刚烷胺用甲醇-1%三氯乙酸(1:1,v/v)提取,经固相萃取柱净化、浓缩后,采用液相色谱-串联质谱法在正离子模式下检测。结果该方法检测限为5.0μg/kg。在1~100μg/kg范围内线性关系良好,相关系数r>0.999。在低、中、高三个添加水平下,该方法的平均回收率为89.0%~98.7%,相对标准偏差为0.3%~2.5%(n=5),变异系数为1.9%~5.5%(n=5)。结论本方法灵敏度高、准确性好、适用于鸡肉中金刚烷胺残留的检测。
Objective To establish a method for the determination of amantadine in chicken by liquid chromatography-tandem mass spectrometry. Methods Amantadine in chicken samples was extracted with methanol-1% trichloroacetic acid (1: 1, v / v) and purified by solid-phase extraction. After concentration, liquid chromatography- tandem mass spectrometry Detection. Results The detection limit of this method was 5.0 μg / kg. In the range of 1 ~ 100μg / kg, the linearity was good with the correlation coefficient r> 0.999. The average recoveries of the method were 89.0% ~ 98.7% with relative standard deviations of 0.3% ~ 2.5% (n = 5), and the coefficients of variation were 1.9% ~ 5.5% 5). Conclusion The method is sensitive, accurate and suitable for the detection of amantadine residues in chicken.