论文部分内容阅读
【目的】Wolbachia是一种广泛存在于节肢动物中的胞内共生细菌,影响寄主的生物学特性。花蓟马Frankliniella intonsa(Trybom)是重要的害虫,对农作物及园林植物造成危害。本研究旨在明确Wolbachia在花蓟马中的感染情况,并分析其与寄主线粒体DNA多样性的关系。【方法】采集中国境内26个花蓟马自然种群,运用多位点序列分型技术(multilocus sequence typing,MLST)对其体内Wolbachia感染率及株系进行分析;利用线粒体COI分子标记研究花蓟马的遗传分化及遗传多样性;通过比较感染和未感染Wolbachia个体COI数据,探究Wolbachia多样性与寄主线粒体DNA多样性之间的关系。【结果】花蓟马中Wolbachia的感染率为0%~60%,共检测到5种Wolbachia株系(w Fint1,w Fint2,w Fint3,w Fint4及w Fint5),均属于B大组且形成一个单系群。Wolbachia感染情况与这些花蓟马种群(除CC,GZ,TA和TY,N<5)的线粒体DNA多样性相关,表现为不感染Wolbachia的种群中线粒体DNA单倍型多样性(Hd)与核苷酸多样性(Pi)均高于感染Wolbachia的种群,且Wolbachia感染率与Hd呈显著负相关(P<0.05)。AMOVA分析表明花蓟马线粒体DNA遗传分化与Wolbachia感染情况有关。【结论】Wolbachia可能在侵染花蓟马种群后出现遗传分化;Wolbachia感染与寄主线粒体DNA多样性有关。
【Objective】 Wolbachia is an intracellular symbiotic bacteria widely found in arthropods and affects the host biological characteristics. Flower thrips Frankliniella intonsa (Trybom) is an important pest that harms crops and garden plants. The aim of this study was to determine the infection status of Wolbachia in the flower thrips and to analyze its relationship with the diversity of host mitochondrial DNA. 【Method】 The natural populations of 26 flower thrips in China were collected and their Wolbachia infection rates and strains were analyzed by multilocus sequence typing (MLST). Mitochondrial COI The genetic diversity and genetic diversity of Wolbachia individuals were investigated. The relationship between Wolbachia diversity and host mitochondrial DNA diversity was explored by comparing the COI data of infected and non-infected Wolbachia individuals. 【Result】 Five Wolbachia strains (w Fint1, w Fint2, w Fint3, w Fint4 and w Fint5) were detected in Wolbachia, which belonged to group B and formed A single line group. The Wolbachia infection was associated with mitochondrial DNA diversity in these flower thrips populations (except CC, GZ, TA and TY, N <5) as shown by differences in mitochondrial DNA haplotype diversity (Hd) and nuclear The nucleotide diversity (Pi) was higher than that of Wolbachia, and Wolbachia infection rate was negatively correlated with Hd (P <0.05). AMOVA analysis showed that the genetic differentiation of mitochondrial DNA in flower thrips was related to Wolbachia infection. 【Conclusion】 Wolbachia may have genetic differentiation after the population of F. occidentalis is infected. Wolbachia infection is related to the diversity of host mitochondrial DNA.