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目的制备载万古霉素硅溶胶(vancomycin-sol,VCM-SOL)涂层缓释系统,探讨其体外抑菌性能。方法采用正硅酸乙酯酸化水解法及溶胶-凝胶法制备载万古霉素(VCM)浓度为20 mg/ml的VCM-SOL涂层钛钢片作为实验组,普通钛钢片作为对照组。利用红外光谱分析系统(FT-IR)来分析实验组的表面基团;选用金黄色葡萄球菌(staphylococcus aureus,S.A)作为实验菌,通过细菌计数进行洗脱液抑菌实验,扫描电镜检测钛钢片表面细菌黏附实验,对比观察实验组和对照组的体外抗菌效果。结果硅溶胶对万古霉素有良好的吸附作用,实验组的洗脱液相对于对照组明显抑制了金黄色葡萄球菌的生长;实验组表面黏附的细菌较为分散,且大部分金黄色葡萄球菌被分解,而对照组表面细菌结构正常,细菌集结成团,易形成生物膜。结论万古霉素以硅溶胶为载体携载到钛合金表面涂层中后,具有良好的体外抗菌活性。
OBJECTIVE To prepare vancomycin-sol (VCM-SOL) coated sustained-release system and investigate its antimicrobial activity in vitro. Methods VCM-SOL coated titanium steel with vancomycin (VCM) concentration of 20 mg / ml was prepared by TEOS acid hydrolysis and sol-gel method as the experimental group, and the common titanium steel slice was used as the control group . The surface groups of the experimental group were analyzed by FT-IR spectroscopy. Staphylococcus aureus (SA) was used as the experimental bacteria, and the bacteriostasis of the elution solution was determined by the bacterial count. The scanning electron microscopy Bacterial adhesion experiments on the surface of the tablets were compared with the antibacterial activity of the experimental group and the control group in vitro. Results Silica sol had a good adsorption effect on vancomycin. The elution of the experimental group significantly inhibited the growth of Staphylococcus aureus compared with the control group. The bacteria adhered to the surface of the experimental group were more dispersed, and most of the Staphylococcus aureus Decomposition, while the control group of bacteria on the surface of normal structure, bacteria assembled into a group, easy to form a biofilm. Conclusion Vancomycin has good antibacterial activity in vitro after being loaded on titanium alloy surface coating with silica sol.