论文部分内容阅读
背景:因糖尿病条件下骨质代谢存在紊乱,对这类骨缺损的修复具有挑战性,研究糖尿病环境下脂肪干细胞的成骨特性将为其在特定环境下的应用提供理论基础。目的:观察高糖、糖基化终末产物对人脂肪干细胞成骨分化能力的影响。法:选取 27.5 mmol/L 高糖、100 mg/L糖基化终末产物体外模拟糖尿病环境,干预人脂肪干细胞成骨分化;实验分为 4 组,每组设立 6 个样本。通过荧光染色检测脂肪干细胞诱导成骨 21 d 时的Ⅰ型胶原表达量,矿化结节染色观测各组中等量脂肪干细胞在14,21,28 d 时矿化结节形成的数量。结果与结论:21 d 时,高糖联合糖基化终末产物组中Ⅰ型胶原荧光强度较正常组低2.76 倍;5 个视野下脂肪干细胞平均矿化结节数量与正常组、单纯高糖组、单纯糖基化终末产物组相比明显下降,差异有显著性意义(P < 0.01)。高糖及糖基化终末产物会抑制脂肪干细胞向成骨方向的同源分化,提示糖尿病环境下,高糖与糖基化终末产物的存在是导致脂肪干细胞成骨分化能力下降的不利因素。
BACKGROUND: Due to the disorder of bone metabolism under the condition of diabetes, the repair of such bone defects is challenging. To study the osteogenic characteristics of adipose-derived stem cells in diabetic environment will provide a theoretical basis for its application in specific environment. Objective: To observe the effect of high glucose and advanced glycation end products on the osteogenic differentiation of human adipose-derived stem cells. Method: 27.5 mmol / L high glucose and 100 mg / L glycosylated end products were used to simulate diabetic environment in vitro to interfere human osteoblast differentiation. The experiment was divided into 4 groups with 6 samples in each group. The expression of type Ⅰ collagen was detected by fluorescence staining at day 21 after osteoblast induction. The number of mineralized nodules in each group was observed at 14,21,28 d by mineralized nodule staining. RESULTS AND CONCLUSION: The fluorescence intensity of type Ⅰ collagen in the group of high glucose and glycosylation end products was 2.76 times lower than that of the normal group on 21 d. The number of average mineralized nodules of adipose-derived stem cells in 5 fields was significantly lower than that of the normal group, Group, simple glycosylation end product group was significantly decreased, the difference was significant (P <0.01). High glucose and advanced glycation end products inhibit the adipogenic differentiation of adipose-derived stem cells into osteoblasts, suggesting that the presence of high glucose and advanced glycation end products (ADs) in adipose tissue is a negative factor leading to decreased osteogenic differentiation of ADSCs .