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目的探讨如何规范过氧化氢低温等离子灭菌生物培养监测过程,准确判断灭菌效果,确保灭菌质量,防止院内感染的发生。方法收集本院2014年过氧化氢低温等离子灭菌生物培养监测的结果,使用CASP-120型过氧化氢低温等离子灭菌器进行检测。采用自含式CASP过氧化氢低温等离子灭菌专用生物指示剂,指示菌为嗜热脂肪芽孢杆菌(ATCC7953),芽孢含量为5×105cfu/支~6×106cfu/支,进行生物培养,并做同步阳性对照监测,检验灭菌效果。结果采样196次生物培养监测,合格率为98.98%,同步监测阳性对照196次,合格率为99.49%。结论操作环节对生物培养监测结果有较大的影响,必须严格规范操作,才能获得有价值的生物培养结果,从真正意义上检测灭菌效果。建议使用新一代过氧化氢低温等离子灭菌生物装置(PCD)进行检测,使检测结果更为准确。
Objective To explore how to standardize the monitoring process of hydrogen peroxide hydrogen plasma sterilization culture to accurately determine the sterilization effect to ensure the quality of sterilization and prevent the occurrence of nosocomial infection. Methods The results of the monitoring of H2O2 low temperature plasma sterilization in our hospital were collected and detected by CASP-120 hydrogen peroxide plasma sterilizer. The self-contained CASP hydrogen peroxide low temperature plasma sterilization biological indicator, the indicator bacteria Bacillus stearothermophilus (ATCC7953), the spore content of 5 × 105cfu / support ~ 6 × 106cfu / support for biological culture, and do Synchronized positive control monitoring, test sterilization. Results 196 samples of biological culture monitoring, the pass rate was 98.98%, simultaneous monitoring of positive control 196 times, with a pass rate of 99.49%. Conclusion The operational aspects of the biological culture monitoring results have a greater impact, we must strictly regulate the operation, in order to obtain valuable biological culture results, the true sense of sterilization. It is recommended to use a new generation of hydrogen peroxide plasma biomonitoring device (PCD) for testing to make the test results more accurate.