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目的 观察成年金黄地鼠左侧视神经眶内切断术后 ,视交叉、视束、外侧膝状体以及上丘内小热休克蛋白 2 7(smallheatshockprotein ,HSP2 7)表达的变化。 方法 免疫组织化学染色法及光密度测定。 结果 在正常对照和手术对照组 ,双侧视交叉、视束、外侧膝状体和上丘未见明显免疫反应物 ,光密度值之间亦无显著差异。而在实验组 ,与左侧相比 ,右侧视交叉、视束、外侧膝状体和上丘免疫组织化学染色明显增强。术后 1周 ,右侧脑内视觉通路可见免疫反应物明显沉着的阳性细胞 ,形态类似星形胶质细胞。统计学分析显示 ,视交叉、外侧膝状体和上丘左、右两侧的光密度差值在术后 1周时最大 ,至术后第 2周迅速降低 ,以后下降趋势减缓 ,到术后 8周仍可见右侧光密度值高于左侧。 结论 一侧视神经切断后 ,对侧视交叉、视束、外侧膝状体和上丘等区域均有HSP2 7表达的增强 ,并可持续至术后 8周之久。提示上述区域HSP2 7表达增强与视觉通路的损伤有关 ,但其发生机理及生物学意义尚待进一步研究。
Objective To observe the changes of the expression of small heatshock protein 27 (HSP27) in optic chiasm, optic canal, lateral geniculate and superior colliculus after orbital severment of left optic nerve in adult golden hamster. Methods Immunohistochemical staining and optical density determination. Results There was no significant difference in optical density between normal control group and surgical control group, bilateral optic chiasm, optic tract, lateral geniculate body and superior colliculus. In the experimental group, compared with the left, the right optic chiasm, visual tract, lateral geniculate body and the superior colliculus immunohistochemical staining was significantly enhanced. One week after operation, the positive cells in the right intracerebral visual pathways were found to be obviously calm with immunoreactive substances. The morphology was similar to that of astrocytes. Statistical analysis showed that the optical density difference between optic chiasm, lateral geniculate body and left and right superior superior colliculus was the largest at 1 week after operation and rapidly decreased at 2 weeks after operation, and then decreased gradually after operation 8 weeks still visible right optical density value higher than the left. Conclusions After the optic nerve is cut off, the expression of HSP27 increases in the lateral cross, the optic tract, the lateral geniculate body and the superior colliculus, and it can persist to 8 weeks after operation. These results suggest that the enhanced expression of HSP27 in the above regions is related to the damage of visual pathway, but its mechanism of occurrence and biological significance remains to be further studied.