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目的探讨二氮嗪对新生大鼠缺氧缺血性脑损伤(HIBD)模型的保护作用。方法70只SD新生鼠随机分为假手术对照组(C组)、缺氧缺血组(HI组)、二氮嗪干预1组(HI之前用药)、干预2组(HI后用药)、干预3组(二氮嗪+5-HD组);HIBD后72h时取各组左脑皮层组织测定线粒体膜微黏度、磷酸腺苷含量,电镜下观察线粒体超微结构。结果HIBD后左脑组织线粒体膜微黏度增加,HI组、干预1组、干预2组及干预3组与C组相比差异均有统计学意义(P<0·01),干预1组降低膜微黏度,与HI组、干预2组及干预3组相比,差异有统计学意义(P<0·01);干预2组及干预3组与HI组相比差异无统计学意义(P>0·05)。HIBD后脑组织ATP含量下降,HI组、干预1组、干预2组及干预3组与C组相比差异均有统计学意义(P<0·01),干预1组与HI组、干预2组及干预3组相比ATP含量增加,差异有统计学意义(P<0·05),干预2组及干预3组与HI组相比差异无统计学意义(P>0·05)。HIBD后HI组、干预2组及干预3组ADP含量下降,HI组及干预3组与C组相比差异有统计学意义(P<0·05);干预1组ADP含量升高,与干预2组、干预3组及HI组相比差异均有统计学意义(P<0·05),但与C组相比差异无统计学意义。HIBD后AMP含量有所增高,HI组及干预3组与C组相比差异有统计学意义(P<0·05)。干预1组和干预2组与C组相比差异无统计学意义。结论HIBD前给予二氮嗪预处理能减少线粒体膜微黏度增加,保护线粒体结构完整性,减少能量的下降,且此作用可被5-HD所抵消,推测其保护作用可能与激活线粒体KATP通道有关。
Objective To investigate the protective effect of diazoxide on hypoxic-ischemic brain damage (HIBD) in neonatal rats. Methods Seventy SD neonatal rats were randomly divided into sham-operation control group (C group), hypoxic-ischemic group (HI group), diazoxide intervention group 1 (before HI administration), intervention group 2 3 groups (diazoxide + 5-HD group). At 72h after HIBD, the mitochondrial membrane microviscosity and phosphorylation of adenosine monophosphate were measured in each group. The ultrastructure of mitochondria was observed under electron microscope. Results The mitochondrial membrane microviscosity increased in HIBD group. There were significant differences in HI group, intervention group 1, intervention group 2 and intervention group 3 compared with those in C group (P <0.01) Microviscosity, compared with HI group, intervention group 2 and intervention group 3, the difference was statistically significant (P <0.01); there was no significant difference between intervention group 2 and intervention group 3 and HI group (P> 0 · 05). Compared with C group, the content of ATP in HIBD group was significantly decreased (P <0.01), intervention group 1 and HI group, intervention group 2 (P <0.05). There was no significant difference between intervention group 2 and intervention group 3 and HI group (P> 0.05). The levels of ADP in HI group, intervention group 2 and intervention group 3 were decreased after HIBD, the difference was statistically significant between HI group and intervention group 3 and C group (P <0.05) There was significant difference between the two groups (P <0.05), the intervention group (3) and the HI group (P <0.05), but there was no significant difference compared with the C group. The content of AMP increased after HIBD, and there was significant difference between HI group and intervention group 3 and C group (P <0.05). There was no significant difference between intervention group 1 and intervention group 2 and group C Conclusion Pretreatment with diazoxide before HIBD can reduce the increase of mitochondrial microviscosity, protect the integrity of mitochondrial structure and decrease the energy loss, which can be offset by 5-HD, suggesting that its protective effect may be related to the activation of mitochondrial KATP channel .