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目的探讨胡桃醌对人卵巢癌SKOV3细胞转移抑制作用。方法实验设对照组、胡桃醌12.5、25.0、50.0、100.0μmol/L组,通过噻唑蓝(MTT)实验检测细胞增殖能力,黏附实验和Transwell实验观察胡桃醌对SKOV3细胞黏附和侵袭能力,Western blot检测基质金属蛋白酶2(MMP-2)和MMP-9蛋白表达变化。结果与对照组比较,胡桃醌12.5、25.0、50.0、100.0μmol/L组SKOV3细胞抑制率分别为(16.8±3.4)%、(39.6±9.7)%、(63.3±13.6)%和(84.5±17.3)%,明显升高,呈剂量依赖性;与对照组比较,25.0、50.0、100.0μmol/L胡桃醌组SKOV3细胞黏附率和侵袭力分别为(49.2±6.5)%、(37.3±2.9)%、(24.7±3.7)%和(0.627±0.064)、(0.419±0.018)、(0.165±0.014),明显下降(P<0.05);与对照组比较,胡桃醌50.0、100.0μmol/L组SKOV3细胞内MMP-2、MM-9表达水平明显下降(P<0.05),呈剂量依赖关系。结论胡桃醌能明显抑制SKOV3细胞黏附能力及侵袭力,其机制可能与抑制细胞内MMP-2、MMP-9表达有关。
Objective To investigate the inhibitory effect of juglone on the metastasis of human ovarian cancer cell line SKOV3. Methods The control group, juglone 12.5, 25.0, 50.0, 100.0μmol / L group, cell proliferation by MTT assay, adhesion and transwell experiments observed juglone SKOV3 cell adhesion and invasion ability, Western blot The changes of MMP-2 and MMP-9 protein expression were detected. Results Compared with the control group, the inhibitory rates of juglone 12.5, 25.0, 50.0 and 100.0 μmol / L were (16.8 ± 3.4)%, (39.6 ± 9.7)%, (63.3 ± 13.6)% and (84.5 ± 17.3) ) Were significantly increased in a dose-dependent manner. Compared with the control group, the adhesion and invasiveness of SKOV3 cells in 25.0,50.0,100.0μmol / L juglone group were (49.2 ± 6.5)% and (37.3 ± 2.9)%, respectively , (24.7 ± 3.7)% and (0.627 ± 0.064), (0.419 ± 0.018) and (0.165 ± 0.014), respectively (P <0.05). Compared with the control group, the SKOV3 cells in Juglone 50.0 and 100.0μmol / The expression of MMP-2 and MM-9 decreased significantly (P <0.05) in a dose-dependent manner. Conclusions Juglone can significantly inhibit the adhesion and invasiveness of SKOV3 cells, which may be related to the inhibition of the expression of MMP-2 and MMP-9 in SKOV3 cells.