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目的研究接受高效抗逆转录治疗(HAART)的HIV-1感染者Th17、Treg的比例及平衡状态,及其与T细胞亚群活化的相关性,以了解Th17/Treg平衡对HAART治疗下免疫活化及疾病进展的影响。方法纳入28例HIV-1感染者(11例接受HAART治疗6个月以上、17例未治疗)及4例健康志愿者,检测并比较其血浆病毒载量、CD4细胞数、Treg、Th17亚群比例,以及HLA-DR、CD38、Ki67等的表达情况。结果 HAART治疗组的Treg及CD25-FoxP3+CD4均高于未治疗组、且表达IL-17的非CD4细胞(CD4-IL-17+)低于未治疗组,差异有统计学意义(P<0.05);Th17/Treg比值略低于未治疗组,差异无统计学意义。HAART组CD8群体中HLA-DR+CD38+、Ki67+的比例低于未治疗组,差异有统计学意义(P<0.05)。Th17比例与HLA-DR+CD38+CD4、HLA-DR+CD38+CD8及Ki67+CD8的比例呈显著正相关(P<0.05)。结论 HAART治疗的感染者高比例的Treg及CD25-FoxP3 CD4可能是其免疫抑制的重要因素。Th17细胞比例与CD8的活化与增殖及CD4的活化明显相关。调节Treg及Th17可能成为辅助治疗的新干预靶点。人类免疫缺陷病毒(human immunodeficiency virus 1,HIV-1)感染及其导致的艾滋病(acquired immune deficiency syndrome,AIDS)是严重危及人类生命健康的重大传染性疾病,迄今为止,公认有效
Objective To investigate the proportion and balance of Th17 and Tregs in HIV-1 infected with HAART and their correlation with T cell subsets activation to understand the role of Th17 / Treg balance in immune activation under HAART And the impact of disease progression. Methods Twenty-eight HIV-1 infected patients (11 HAART treated for more than 6 months and 17 untreated) and 4 healthy volunteers were enrolled in the study. Plasma viral load, CD4 cell count, Treg and Th17 subsets Ratio, and the expression of HLA-DR, CD38, Ki67 and so on. Results The levels of Treg and CD25-FoxP3 + CD4 in HAART group were significantly higher than those in untreated group, and the CD4-IL-17 positive cells expressing IL-17 were significantly lower than those in untreated group (P < 0.05). The Th17 / Treg ratio was slightly lower than the untreated group, the difference was not statistically significant. The proportion of HLA-DR + CD38 + and Ki67 + in HA group was lower than that in untreated group, the difference was statistically significant (P <0.05). There was a significant positive correlation between the proportion of Th17 and HLA-DR + CD38 + CD4, HLA-DR + CD38 + CD8 and Ki67 + CD8 (P <0.05). Conclusion The high proportion of Treg and CD25-FoxP3 CD4 in infected patients with HAART may be an important factor of immunosuppression. Th17 cell ratio and CD8 activation and proliferation and CD4 activation was significantly correlated. Regulating Treg and Th17 may be new interventional targets for adjuvant therapy. Human immunodeficiency virus 1 (HIV-1) infection and its AIDS-related disease (AIDS) are serious infectious diseases that seriously endanger human life and health. So far, it has been recognized as effective