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目的:研究水芹醋酸乙酯提取物(Sf-Et)对大鼠脑缺血-再灌注损伤的保护作用,并探讨其机制。方法:取大鼠50只,随机分为5组,即假手术组、脑缺血-再灌注模型组、Sf-Et(200,100mg.kg-1)剂量组、维拉帕米(Ver)(0.2mg.kg-1)、阳性组,每组10只。采用阻断大鼠双侧锁骨下动脉及颈总动脉的方法,制备大鼠脑缺血30min-再灌注60min损伤模型,观察Sf-Et对模型大鼠脑缺血前、缺血30min、再灌注60min后的脑电图(EEG)、血清中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、胞浆内钙离子(Ca2+)浓度及脑组织病理变化的影响。结果:Sf-Et各剂量组均可促进缺血后EEG波幅的恢复;提高血清中SOD活性;降低MDA含量及胞浆内Ca2+浓度;减轻脑组织的病理变化。结论:Sf-Et对脑缺血-再灌注损伤有一定保护作用,其机制可能与其抗自由基,抑制钙超载有关。
Objective: To study the protective effect of ethyl acetate extract of watercress (Sf-Et) on cerebral ischemia-reperfusion injury in rats and to explore its mechanism. METHODS: Fifty rats were randomly divided into 5 groups: sham operation group, cerebral ischemia-reperfusion model group, Sf-Et (200, 100 mg.kg-1) dose group, verapamil (Ver) ( 0.2 mg.kg-1), positive group, 10 in each group. A rat model of cerebral ischemia 30 min-reperfusion 60 min was prepared by blocking the bilateral subclavian and common carotid arteries of rats. The effects of Sf-Et on cerebral ischemia, ischemia 30 min, and reperfusion were observed in model rats. Electroencephalogram (EEG), serum superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, cytoplasmic calcium ion (Ca2+) concentration, and pathological changes of brain tissue after 60 min. RESULTS: All doses of Sf-Et could promote recovery of EEG amplitude after ischemia, increase SOD activity in serum, decrease MDA content and cytoplasmic Ca2+ concentration, and reduce pathological changes in brain tissue. Conclusion: Sf-Et has a certain protective effect on cerebral ischemia-reperfusion injury, and its mechanism may be related to its anti-free radical and inhibition of calcium overload.