论文部分内容阅读
选40名健康的妇女,在妊娠16周左右进行诊断性的羊膜腔穿刺。吸烟者日吸15支以上。对照组年龄、产次均与吸烟组配对。穿刺前详细询问了妊娠期间(尤其是近期)与烟草和其它可能的诱变剂接触的情况。计算了穿刺前48小时中所吸烟的焦油含量。羊水离心后将上清液贮存于-20℃下,然后融化,冰冻干燥,并溶于无菌蒸馏水中,使浓缩25倍。按标准Ames试验方法,用TA_(98)和TA_(100)加S 9进行致突变性检定。因样品量有限,用TA_(100)仅测试了5名吸烟者和7名非吸烟者的
Forty healthy women were selected for diagnostic amniocentesis around 16 weeks gestation. Smokers inhale more than 15 days. Control group, age, parity and smoking group paired. Detailed information about the condition of contact with tobacco and other possible mutagens during pregnancy (especially in the near future) before puncture. The tar content of cigarettes smoked during 48 hours before puncture was calculated. After centrifugation of the amniotic fluid, the supernatant was stored at -20 ° C, thawed, lyophilized, and dissolved in sterile distilled water to concentrate 25 times. According to the standard Ames test method, TA_ (98) and TA_ (100) plus S 9 mutagenicity test. Due to the limited sample size, only 5 smokers and 7 non-smokers were tested with TA_ (100)