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按Selye法结扎Wistar大鼠冠状动脉左前降支，复制早期心肌缺血（EMI）模型。根据心肌缺血不同时间（15min、30min、1h、2h、3h）分5个实验组，另设1个对照组；每组取心尖部及其邻近的心肌组织制成石蜡切片，进行HE、链霉系合素生物素过氧化物酶（SAB）染色。结果表明：早期心肌缺血30min组，缺血区心肌即出现肌红蛋白（Mb）缺失和纤维蛋白原（Fg）染色增强。随着缺血时间延长，Mb缺失或Fg染色增强范围扩大，程度加重，从心内膜下向外层心肌发展呈“波浪式推进现象”，二者在心肌缺血过程中的变化规律相同，灵敏度相似，但Fg易受血液污染而致其可靠性较Mb差。在注意血液污染的前提下，SABC－Fg技术为诊断EMI提供了新的途径。
The left anterior descending coronary artery of Wistar rats was ligated by Selye method to replicate the model of early myocardial ischemia (EMI). According to myocardial ischemia at different times (15min, 30min, 1h, 2h, 3h), five experimental groups were divided into two groups. One group was given another control group. The apex and its adjacent myocardium were paraffin- Mycotoxin biotin peroxidase (SAB) staining. The results showed that in the early 30min myocardial ischemia, myoglobin (Mb) deletion and fibrinogen (Fg) staining increased in the ischemic myocardium. With the prolongation of ischemic time, Mb deletion or Fg staining enhanced the scope of expansion, increasing degree, from the endocardium to the outer layer of myocardial development showed a “wave-like advance”, the two changes in the process of myocardial ischemia in the same, Sensitivity is similar, but Fg is more susceptible to blood contamination than Mb. The SABC-Fg technique provides a new way to diagnose EMI while paying attention to blood contamination.