论文部分内容阅读
目的:研究黄芪及其拆分组分(黄酮、皂苷、多糖、水溶液)对脾虚水湿不化大鼠十二指肠矿物质吸收相关基因表达谱的影响,阐明黄芪性味归属。方法:高脂低蛋白饮食与力竭游泳法建立脾虚水湿不化大鼠模型,黄芪及其拆分组分灌胃2周。Agilent大鼠基因表达谱芯片检测各组大鼠十二指肠基因表达谱变化,筛选差异基因,并进行生物信息学分析。荧光实时定量PCR验证Trpm6、Reg1α基因表达。结果:与模型组比较,黄芪水煎液组688个差异基因,GO功能分类涉及金属离子转运、阳离子平衡等;黄酮组1 204个差异基因,涉及钠、钾、铁、铜等离子运输;皂苷组1 361个差异基因,涉及二价金属离子外流、铁离子平衡、铜离子解毒等;多糖组3 181个差异基因,涉及阳离子运输、铁离子运输等;水溶液组2 419个差异基因,涉及镁离子应答、阳离子运输等。KEGG分析结果显示,各给药组生物学通路均涉及矿物质吸收,据差异基因富集由高到低为多糖组>黄酮组>水溶液组>皂苷组>黄芪水煎液组。荧光实时定量PCR验证结果和芯片结果一致。结论:黄芪及其拆分组分通过调节模型大鼠十二指肠与铁、铜、镁、磷等吸收相关基因,维持机体离子平衡,体现其性温、味甘,多糖组分作用更好。
Objective: To study the effect of Astragalus membranaceus and its fractions (flavonoids, saponins, polysaccharides, aqueous solution) on the expression profiles of mineral absorption related genes in duodenum of rats with spleen-asthenia-dampness syndrome. Methods: A rat model of spleen deficiency and water wetting was established by high-fat and low-protein diet and exhaustive swimming. Astragalus membranaceus and its fractions were orally administered for 2 weeks. The gene expression profiles of rat duodenum were detected by Agilent rat gene expression microarray. The differential genes were screened and bioinformatics analysis was performed. Real-time quantitative PCR confirmed the expression of Trpm6 and Reg1α. Results: Compared with the model group, 688 differential genes in GO, decoction of GO were involved in metal ion transport and cation balance, while 1 204 differential genes in flavonoids group were related to plasma transport of sodium, potassium, iron and copper; 1 361 differential genes involved in the outflow of bivalent metal ions, iron ion balance and copper ion detoxification; 3 181 differentially expressed genes in the polysaccharide group involved in cation transport and ferrous ion transport; and 2 419 differential genes in aqueous solution group involving magnesium ions Response, cation transport and so on. The results of KEGG analysis showed that the mineral pathways were all involved in the biological pathways of the administration groups, and the polysaccharide group> flavonoid group> aqueous solution group> saponin group> astragalus root decoction group according to the difference gene enrichment from high to low. Fluorescence real-time quantitative PCR validation results and chip results. Conclusion: Astragalus membranaceus and its fractions can regulate the ion balance of duodenum, iron, copper, magnesium and phosphorus, and maintain the ion balance of the body. .