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为探讨最近新发现的干酪乳杆菌Qian(Lactobacillus casei Qian,LC-Qian)和京尼平苷组合对胃损伤预防效果的增强作用,比较分析LC-Qian(0.5×10~8 CFU/kg,以体质量计,下同)、京尼平苷(50 mg/kg)以及LC-Qian(0.5×10~8 CFU/kg)+京尼平苷(50 mg/kg)对盐酸/乙醇诱导胃损伤的预防效果。LC-Qian+京尼平苷(组合组)能减少胃损伤面积和胃液量,同时提高胃液pH值,能使这些指标接近正常组并且与模型组显著不同。相对于模型组,组合组能降低细胞因子白细胞介素-6(interleukin-6,IL-6)、IL-12、肿瘤坏死因子α和γ-干扰素的水平,同时降低血清胃动素、P物质、内皮素-1的水平和提高生长激素抑制素、血管活性肠肽的水平。相比模型组,在心脏、肝脏、肾脏和胃组织中组合组表现出更高的NO含量,但是这些含量低于正常组。通过逆转录聚合酶链式反应分析观察到:相对于对照组小鼠,组合组能够上调神经元型一氧化氮合酶、内皮型一氧化氮合酶、核因子κB抑制蛋白α的mRNA表达和下调诱导型一氧化氮合成酶、核因子κB表达,同时以上这些表达强度与正常组较为接近。通过这些结果可以看到LC-Qian和京尼平苷联合使用具有最好的胃损伤预防效果,LC-Qian能够提高京尼平苷的胃损伤预防效果,该组合可作为一种新的参考方法用于预防胃损伤。
To investigate the effect of the newly discovered Lactobacillus casei Qian (LC-Qian) and geniposide combination on the prevention of gastric injury, we compared the effects of LC-Qian (0.5 × 10-8 CFU / kg, Hydrochloric acid / ethanol-induced gastric injury (50 mg / kg) and LC-Qian (0.5 × 10-8 CFU / kg) plus geniposide (50 mg / Prevention effect. LC-Qian + Geniposide (combination group) can reduce the area of gastric injury and gastric juice, while increasing the pH value of gastric juice can make these indicators close to the normal group and significantly different from the model group. Compared with the model group, the combination group can reduce the levels of interleukin-6 (IL-6), IL-12, TNF-α and IFN-γ and decrease the levels of serum motilin, P Substance, endothelin-1 levels and increase somatostatin, vasoactive intestinal peptide levels. The combination group showed higher NO content in the heart, liver, kidney and stomach tissue than the model group, but these contents were lower than those in the normal group. Reverse transcriptase-polymerase chain reaction analysis showed that the combination group was able to up-regulate the mRNA expression of neuronal nitric oxide synthase, endothelial nitric oxide synthase and nuclear factor kappa B inhibitor alpha relative to the control mice Down-regulated the expression of inducible nitric oxide synthase and nuclear factor κB, at the same time these above expression intensity were close to normal group. From these results, it can be seen that LC-Qian and geniposide have the best effect of preventing gastric injury, LC-Qian can improve the preventive effect of geniposide on gastric injury, and the combination can be used as a new reference method Used to prevent stomach damage.