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目的:比较骨髓间充质干细胞移植和骨髓动员对急性心肌梗死再通模型心功能作用,及两者心肌及血管再生作用。方法:将小型猪模型分为3组,对照组(5头),骨髓动员组(6头),细胞移植组(8头)。心肌梗死再通模型制作后,骨髓动员组予人重组粒细胞集落刺激因子静脉注射1天,皮下注射连续5天,细胞移植组予骨髓间充质干细胞(4—8)×106经梗死相关冠脉注入。3周后观察以下指标:(1)超声心动图检测左室射血分数。(2)免疫组织化学染色,行不同区域血管计数,并观察增殖期内皮细胞和心肌细胞或再生心肌细胞。结果:(1)骨髓动员组和细胞移植组心功能明显改善。(2)细胞移植组在心肌梗死周围区可见分散分布的BrdU标记并分化为心肌细胞的移植细胞,骨髓动员组中亦观察到处于增殖期的心肌细胞;细胞移植组和骨髓动员组均可见大量新生血管,但二者分布区域不同。结论:骨髓动员及骨髓间充质干细胞移植均可明显改善心肌梗死后心功能,二者可促进心肌细胞再生,二者对不同区域的血管再生作用不同。
OBJECTIVE: To compare the cardiac function of bone marrow mesenchymal stem cells transplantation and bone marrow mobilization on reperfusion model of acute myocardial infarction and the effects of myocardial and angiogenesis. Methods: Mini-pigs were divided into three groups: control group (5 heads), bone marrow mobilization group (6 heads) and cell transplantation group (8 heads). After the myocardial infarction recanalization model was established, the bone marrow mobilization group was given human recombinant granulocyte colony-stimulating factor intravenously for 1 day and subcutaneously for 5 consecutive days. The bone marrow mesenchymal stem cells (4-8) Pulse injection. After 3 weeks, the following indicators were observed: (1) echocardiography to detect left ventricular ejection fraction. (2) Immunohistochemical staining, blood vessel count in different regions, and observe the proliferation of endothelial cells and cardiomyocytes or cardiomyocytes. Results: (1) The cardiac function of bone mobilization group and cell transplantation group was significantly improved. (2) In the cell transplantation group, scattered BrdU labeled cells were observed in the peripheral area of myocardial infarction and differentiated into cardiomyocyte-derived cells. In the bone marrow mobilization group, cardiomyocytes were also observed in the proliferative phase. In the cell transplantation group and the bone marrow mobilization group, Neovascularization, but the two regions are different. CONCLUSION: Bone marrow mobilization and bone marrow mesenchymal stem cell transplantation can significantly improve cardiac function after myocardial infarction, both of which can promote cardiomyocyte regeneration. The two have different effects on the regeneration of blood vessels in different regions.