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目的初步研究淫羊藿苷对人牙周膜细胞(human periodontal ligament cells,h PDLC)增殖以及在内毒素(Lipopolysaccharide,LPS)作用下淫羊藿苷对h PDLC白细胞介素6(IL-6)表达的影响。方法组织块法原代培养并鉴定h PDLC,采用MTT方法检测淫羊藿苷(0、10-5、10-6、10-7、10-8、10-9、10-10mol/L)作用下h PDLC增殖的变化;利用PCR、ELISA方法检测淫羊藿苷对在LPS刺激下的人牙周膜细胞IL-6分泌的情况。结果一定浓度范围下(10-6~10-7mol/L)淫羊藿苷对h PDLC的增殖具有积极作用;在10μg/m L LPS作用下h PDLC的IL-6表达增多、活性增强,加入10-6mol/L淫羊藿苷处理后,对此加强效果有一定的抑制作用。结论在一定浓度范围作用下淫羊藿苷可促进h PDLC的增殖;同时,淫羊藿苷对LPS导致h PDLC的IL-6表达增强有一定的抑制作用。
Objective To study the effects of icariin on the proliferation of human periodontal ligament cells (h PDLC) and the effects of icariin on the expression of interleukin 6 (IL-6) in h PDLC under the action of lipopolysaccharide (LPS) The impact of expression. Methods Primary culture and identification of h PDLC were performed by tissue block method. The effects of icariin (0,10-5,10-6,10-7,10-8,10-9,10-10 mol / L) were determined by MTT assay H PDLC proliferative changes; PCR, ELISA method to detect icariin on LPS-stimulated human periodontal ligament cells IL-6 secretion. Results Icariin had a positive effect on the proliferation of h PDLC in a certain concentration range (10-6 ~ 10-7mol / L). IL-6 expression was increased and the activity was increased in h PDLC treated with 10μg / mL LPS. After 10-6mol / L icariin treatment, this effect has a certain degree of inhibition. Conclusions Icariin can promote the proliferation of h PDLC under certain concentration range. In addition, icariin can inhibit the expression of IL-6 of h PDLC to a certain extent by LPS.