目的　建立用高效液相色谱法测定复方制剂中戊酸雌二醇和炔诺酮含量的方法。方法　用氰基键合硅胶为固定相 ,乙腈%D水 ( 4 7∶5 3)为流动相 ,黄体酮为内标 ,UV检测波长为 2 80nm。结果　该方法回收率为戊酸雌二醇 10 0 .6 % ,RSD =0 .9% (n =9) ,炔诺酮 10 0 .2 % ,RSD =0 .8% (n =9) ;测定了三批样品 ,结果满意。结论　该法不需经复杂的样品处理 ,简便、快速 ,准确可靠 ,可作为该复方制剂的含量测定方法。 Objective To establish a method for the determination of estradiol valerate and norethindrone acetate in compound preparations by high performance liquid chromatography. Methods Cyano-bonded silica gel was used as stationary phase. Acetonitrile-D water (4 7:53) was used as the mobile phase, and progesterone was used as internal standard. UV detection wavelength was 280 nm. Results The recovery rate of estradiol valerate was 100.6%, RSD = 0.9% (n = 9), norethisterone 0.2%, RSD = 0.8% (n = 9). Three batches of samples were measured and the results were satisfactory. Conclusion The method does not require complex sample processing, and is simple, rapid, accurate and reliable, and can be used as a method for the determination of the compound preparation.