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本工作采用肝部分切除(PH)后96h大鼠的离体肝细胞,以细胞存活率、细胞内K~+漏出量、介质中GPT、GOT含量和细胞膜微粘度的改变作为指标,观察其抗CCl_4损伤作用。结果如下:(1)细胞温育3h或5h(介质中含5μl/ml,CCL_4)后PH组细胞存活率明显高于假手术组,介质中GPT和GOT含量亦明显降低。(2)细胞培养24h后换含CCL_4(15mmol/L)的新鲜培养液再培养20min,PH组细胞内K~+漏出量明显低于假手术组。(3)PH组细胞膜微粘度明显低于假手术组。这些结果提示大鼠离体再生肝细胞膜稳定性增高可能有抗CCl_4损伤的作用。
In this study, ex vivo hepatocytes were isolated from rat liver after partial hepatectomy (PH) for 96 hours. The survival rate, intracellular K ~ + leakage, GPT, GOT content and microviscosity in the medium were measured as indicators CCl 4 injury. The results were as follows: (1) The cell viability in PH group was significantly higher than that in sham operation group after incubation for 3h or 5h (5μl / ml, CCL_4 in medium), and the content of GPT and GOT in the medium was also significantly decreased. (2) The culture medium containing CCL_4 (15mmol / L) was cultured for another 20min after 24h incubation, and the amount of K ~ + leakage in PH group was significantly lower than that in sham operation group. (3) The microviscosity of cell membrane in PH group was significantly lower than that in sham operation group. These results suggest that the stability of rat liver regeneration membrane regeneration may have anti-CCl 4 damage.