论文部分内容阅读
利用气相沉积技术制备TiO2凝胶膜,将辣根过氧化酶固定在巯基丁二酸铜(II)(CuL-SH)自组装单分子层修饰金电极表面,制得测定H2O2的电流型生物传感器.金电极表面的CuL-SH同时作为电子媒介体.研究了各种因素如pH、工作电位等对传感器响应电流的影响.传感器对H2O2的还原显示出快速电催化响应(<10s),计时电流法测定H2O2的线性范围为2.2μmol/L-0.6mmol/L(R=0.999),检出限为1.0×10-6mol/L.测得酶催化动力学参数米氏常数KMapp=0.56mmol/L.传感器的稳定性好,60d其响应值仍保持90%.对其灵敏度和选择性进行了研究,并应用于实际样品测定.
Titania gel films were prepared by vapor deposition technique and immobilized on horseradish peroxidase (CuL-SH) self-assembled monolayer to immobilize the gold electrode surface. The current-type biosensor for H2O2 determination The CuL-SH on the surface of gold electrode was used as the electronic medium at the same time.The effect of various factors such as pH and working potential on the response current of the sensor was investigated.The sensor showed rapid electrocatalytic response (<10s) to H2O2 reduction, The linear range of the determination of H2O2 was 2.2μmol / L-0.6mmol / L (R = 0.999) and the detection limit was 1.0 × 10-6mol / L. The Km of the enzyme catalytic kinetic parameters KMapp = 0.56mmol / L The stability of the sensor is good, and its response value remains at 90% after 60 days.The sensitivities and selectivity of the sensor are studied and applied to the actual sample determination.