论文部分内容阅读
目的:探讨转录因子激活蛋白1(AP-1)和核转录因子κB(NF-κB)在肝细胞癌(HCC)组织中的表达及其临床意义。方法:采用链菌素抗生物素-过氧化物酶法(SP)检测50例HCC组织和癌旁正常肝组织中AP-1和NF-κB的表达,并分析两者与HCC临床病理因素的关系。结果:HCC组织中AP-1(c-Jun/c-Fos)和NF-κB阳性表达率均明显高于癌旁肝组织,差异有统计学意义,P<0.01。Ac-Jun和c-Fos蛋白的表达与有无门静脉癌栓、淋巴结转移明显相关,P<0.05。NF-κB蛋白表达与病理分级明显相关,P<0.05。HCC组织中AP-1(c-Jun/c-Fos)和NF-κB表达呈正相关,r值分别为0.556和0.538,P值均<0.01。结论:AP-1、NF-κB与HCC的发生有关,且在HCC的发生发展过程中起着不同程度的作用;NF-κB可能通过上调AP-1的表达和活性而促进HCC的浸润和转移。
Objective: To investigate the expression of AP-1 and NF-κB in hepatocellular carcinoma (HCC) tissues and their clinical significance. Methods: The expression of AP-1 and NF-κB in 50 HCC tissues and adjacent normal liver tissues were detected by streptavidin-peroxidase (SP) method. The relationship between them and clinicopathological factors relationship. Results: The positive rate of AP-1 (c-Jun / c-Fos) and NF-κB in HCC tissues were significantly higher than that in adjacent liver tissues, the difference was statistically significant, P <0.01. The expression of Ac-Jun and c-Fos was significantly correlated with the presence of portal vein tumor thrombus and lymph node metastasis (P <0.05). NF-κB protein expression and pathological grade were significantly correlated, P <0.05. There was a positive correlation between the expression of AP-1 (c-Jun / c-Fos) and NF-κB in HCC tissues, with r values of 0.556 and 0.538, respectively, with P values <0.01. CONCLUSION: AP-1 and NF-κB are involved in the development of HCC and play a role in the development of HCC. NF-κB may promote the invasion and metastasis of HCC by up-regulating the expression and activity of AP-1 .