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目的为了观察正常灰鼠和卡铂(Carboplatin)耳中毒灰鼠耳蜗单个疆孔(habenulaperforata)内的神经纤维数量。方法12只灰鼠中6只按100mg/kg腹腔注射卡铂2次,6只作正常对照,3个月后处死,沿蜗轴平面作半薄切片,甲苯胺蓝-美蓝染色后光镜观察,组间统计学比较采用t检验。结果正常灰鼠的神经纤维数量在耳蜗不同部位有所不同,其中在底回起始段每个疆孔中通过93.7±12.2(x±s,下同)根神经纤维,底回末段为181.5±30.4,第二回中段为129.7±27.8,顶回中段为75.8±13.2。应用加倍大剂量卡铂将内毛细胞和I型螺旋神经元全部破坏后,疆孔内残存的神经纤维数仅占正常值的七分之一。结论疆孔内七分之六的神经是支配内毛细胞的I型神经元。
Objective To observe the number of nerve fibers in habenulaperforata of normal squirrel and Carboplatin ototoxic squirrel. Methods Six of 12 squirrels were injected intraperitoneally with carboplatin twice at 100 mg / kg, 6 as normal control, 3 months later sacrificed and semi-thin section along the plane of the worm. Toluidine blue-methylene blue staining light microscope Observation, statistical comparison between groups using t test. Results The number of nerve fibers in normal squirrels was different in different parts of the cochlea. The root of each nerve root in the initial stage of end-bottom passage was 93.7 ± 12.2 (x ± s, the same below) 181.5 ± 30.4 in the final segment, 129.7 ± 27.8 in the second, and 75.8 ± 13.2 in the middle of the top. Application of doubled large doses of carboplatin internal hair cells and I-type spiral neurons destroyed, the number of residual fibers in the villus hole accounted for only one-seventh of normal. Conclusion Six-sixths of the nerves in the vault are type I neurons that innervate hair cells.