目的评价人工合成三糖抗原在结核病血清诊断的应用前景。方法用人工合成的结核杆菌酚糖酯三糖抗原ＴｂＮＴＰＢＳＡ（ｎａｔｕｒａｌｔｒｉｓａｃｃｈａｒｉｄｅｐｈｅｎｙｌｐｒｏｐｉｏｎａｔｅｂｏｖｉｎｅｓｅｒｕｍａｌｂｕｍｉｎ）作抗原，用ＥＬＩＳＡ法检测了４０例结核患者，５２个健康对照的血清标本。结果本方法的特异性、敏感性分别为：检测抗ＴｂＮＴＰＢＳＡＩｇＧ抗体时为９４２％和２００％；检测抗ＴｂＮＴＰＢＳＡＩｇＭ抗体时为７９６％和４５０％。结论该抗原的特异性、敏感性似不理想，有待进一步改进 Objective To evaluate the application of synthetic trisaccharide antigen in the diagnosis of tuberculosis. Methods Thirty human tuberculosis patients and 52 healthy controls were detected by ELISA using Tb-NTPBSPBSA (natural triglycerinphenylpropionatebovine serumalbumin), a synthetic TB antibody against Mycobacterium tuberculosis. Results The specificity and sensitivity of this method were: 94  2% and 20  0% for detecting antiTbNTPBSAIgG antibody, 796% for detecting antiTbNTPBSAIgM antibody, And 45  0%. Conclusion The specificity and sensitivity of the antigen is not ideal, need to be further improved