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目的探讨干扰素(Interferon,IFN)对ICR乳鼠感染肠道病毒71型(Enterovirus 71,EV71)的保护作用。方法将FY23-EV71感染经重组人IFNα1b和IFNλ1预处理的Vero和KMB17细胞,微量滴定法检测病毒滴度;取ICR乳鼠分为PBS对照组、EV71对照组和IFN保护组,临床观察2周,取乳鼠组织,观察病理学变化,并检测病毒含量。结果两种IFN对KMB17细胞和Vero细胞感染EV71均有保护作用,且IFNα1b的保护作用优于IFNλ1;IFN保护组乳鼠较EV71对照组乳鼠在临床表现、体重、病理变化和组织病毒含量等方面均表现出对EV71感染的保护作用。结论 IFN能抑制EV71的增殖,并对病毒致病性和组织嗜性有一定的影响。
Objective To investigate the protective effect of Interferon (IFN) on enterovirus 71 (EV71) in ICR suckling mice. Methods Vero and KMB17 cells pretreated with recombinant human IFNα1b and IFNλ1 were infected by FY23-EV71. The viral titer was detected by microtiter method. The ICR suckling mice were divided into PBS control group, EV71 control group and IFN protection group. , Take the rat tissue, observe the pathological changes, and detect the virus content. Results Both IFNs had protective effects on EV71 cells infected with KMB17 cells and Vero cells, and the protective effect of IFNα1b was better than that of IFNλ1. Compared with EV71 control group, the protective effect of the two IFNs on EV71 mice was better than that of EV71 control group in clinical manifestations, body weight, pathological changes and virus content Both showed protective effects against EV71 infection. Conclusion IFN can inhibit the proliferation of EV71 and have certain effects on pathogenicity and tropism of virus.