论文部分内容阅读
目的：研究胃、大肠癌与端粒酶表达活性及ＤＮＡ倍体的关系。方法：端粒重复扩增－酶联免疫吸附法（ＴＲＡＰ－ＥＬＩＳＡ法）检测肿瘤及无瘤残端粘膜端粒酶活性，流式细胞术检测肿瘤的ＤＮＡ倍体。结果：肿瘤瘤体端粒酶阳性者占８４．０％（４２／５０），无瘤残端酶阳性者占２．０％（１／５０）；端粒酶阳性肿瘤中异倍体肿瘤占５２．３８％（２２／４２），而端粒酶阴性肿瘤无异倍体出现；端粒酶阳性瘤体平均直径 ６．５ｃｍ，阴性瘤体平均直径 ３． ９ｃｍ；端粒酶阳性肿瘤淋巴转移率为 ５９.５％（２５／４２），阴性者转移率为 １２． ５％（１／８），四者差别均有显著意义， Ｐ值分别为： Ｐ＜０．００５，Ｐ＜０．０５，Ｐ＜０．００２，Ｐ＜０．０５。结论：胃癌及大肠癌端粒酶活性升高，酶阳性肿瘤异倍体发生率高，且瘤体大，淋巴转移率高，说明端粒酶的激活在恶性肿瘤的发生发展中是重要因素，并且与疾病的进程相关。
Objective: To study the relationship between gastric cancer, colorectal cancer, telomerase expression activity and DNA ploidy. Methods: The telomerase activity of tumor and tumor-free stump mucosa was detected by TRAP-ELISA method. The DNA ploidy of tumor was detected by flow cytometry. Results: The tumor telomerase-positive patients accounted for 84.0% (42/50), the tumor-free stump-positive patients accounted for 2.0% (1/50); the telomerase-positive tumors accounted for aneuploid tumors. 52.38% (22/42), while no ploidy appeared in telomerase-negative tumors; the average diameter of telomerase-positive tumors was 6.5cm, and the average diameter of negative tumors was 3. 9cm; Lymphatic metastasis rate of telomerase-positive tumor was 59.5% (25/42), and the metastasis rate was negative 12. 5% (1/8), the difference between the four were significant, P values were: P <0.005, P <0.05, P <0.002, P <0.05. Conclusion: The telomerase activity is increased in gastric cancer and colorectal cancer, and the incidence of aneuploidy in enzyme-positive tumors is high. The tumor is large and the lymphatic metastasis rate is high, indicating that telomerase activation is an important factor in the development of malignant tumors. And it is related to the course of the disease.