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目的 了解广东地区GBV -C/HGV 5′NCR区的序列变异情况。方法 以GBV -C和HGV的 5′NCR区完全同源的序列设计合成引物用于套式RT -PCR。从广东地区的 10例GBV -C/HGV感染者血中扩增了 10个序列 ,分别克隆至pUC19或pT -Adv载体。以 3 5S标记的双脱氧链末端终止法对这 10个序列分别进行正反两个方向的序列测定。结果 10株序列相互间同源性最大为 10 0 % ,最小为 84 4%。与Genbank所载 3个标准株的相应序列比较 ,其同源性最大为89 4% ,最小为 83 9%。与国内周育森报道的相应序列比较 ,两者之间的同源性最大为 96 1% ,最小为 85 0 %。比较还发现 ,这 10株序列的起始处有一段 3 8nt的区域与上述标准序列相比变异比较大 ,其与标准序列的同源性最高只有 79 0 % ,最低为 60 5 %。其中 9株相互间的这一 3 8nt序列的同源性则高达 92 %～ 10 0 %。结论 广东地区流行的庚型肝炎病毒大多带有HGV的特殊点。少数为GBV -C
Objective To understand the sequence variation of 5 ’NC region of GBV C / HGV in Guangdong. Methods The synthetic primers were designed for nested RT-PCR using sequences that were completely homologous to the 5’NCR region of GBV-C and HGV. Ten sequences were amplified from blood of 10 patients with GBV-C / HGV infection in Guangdong and cloned into pUC19 or pT-Adv vector respectively. The 10 5 sequences were sequenced in both forward and reverse directions by 3 5S labeled dideoxy chain termination method. Results The homology of the 10 sequences with each other was 100% and the lowest was 84 4%. Compared with the corresponding sequences of the three standard strains contained in Genbank, the homology was up to 89 4% and the minimum was 83 9%. Compared with the corresponding sequences reported by Zhou Yushan in China, the homology between the two was 96 1% at the maximum and 85 0% at the minimum. Comparisons also showed that a region of 38 nt at the beginning of the 10 sequences showed a large variation compared with the above standard sequence, and the highest homology with the standard sequence was only 79.0% and the lowest was 60.5%. Among them, the homology of the 3 8 nt sequences among 9 strains is as high as 92% to 100%. Conclusion Most hepatitis G viruses in Guangdong have the special point of HGV. A few are GBV -C