目的获得胰腺癌重组噬菌体抗体筛检及鉴定的靶抗原．方法用ｐｒｏｔｅｉｎＡｓｅｐｈａｒｏｓｅ４Ｂ柱纯化的单抗ＳＣ６吸附到ＣＮＢｒｓｅｐｈａｒｏｓｅ４Ｂ特异亲合层析柱上，分离出ＳＣ６抗原，再用ＳＤＳＰＡＧＥ，Ｗｅｓｔｅｒｎｂｌｏｔｉｎｇ、免疫化学和氨基酸自动分析仪等检测其有关的特性．结果此抗原在ＳＤＳＰＡＧＥ，Ｗｅｓｔｅｒｎｂｌｏｔｉｎｇ上显示一条带，Ｍｒ６７００００，性质属糖蛋白，其中含１７种氨基酸，与ＣＡ１９９等多种抗原不同，在胰腺癌组织中的阳性表达率为９１２％．结论该抗原是一种与胰腺癌相关的新肿瘤标志物，这为其分子克隆等研究提供了新的抗原蛋白． Objective To obtain target antigens for screening and identification of pancreatic cancer recombinant phage antibodies. Methods The monoclonal antibody SC6 purified on proteinAsepharose4B column was adsorbed onto CNBrsepharose4B-specific affinity chromatography column to isolate SC6 antigen. The relevant characteristics were detected by SDSPAGE, Western blotting, immunochemistry and amino acid automatic analyzer. . Results The antigen showed a band on the SDS-PAGE, Western blotting, Mr670000, the property is a glycoprotein, which contains 17 kinds of amino acids, and different antigens such as CA19  9 and the positive expression rate in pancreatic cancer is 912 %. Conclusion This antigen is a new tumor marker associated with pancreatic cancer, which provides a new antigenic protein for molecular cloning studies.