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目的:观察自拟中药复方“益肾蠲湿合剂”对肾小球系膜细胞(glomerular mesangial cell,GMC)增殖的影响,并进一步探讨其作用机理。方法:将1×10-3、1×10-2和1×10-1mg/mL的“益肾蠲湿合剂”提取液作用于体积分数10%血清的DMEM培养的GMC,分别用MTT和Western Blot方法检测细胞的增殖和磷酸化ERK1/2的表达。结果:1×10-2和1×10-1mg/mL“益肾蠲湿合剂”可显著抑制GMC的增殖,抑制率在12、24和36 h分别为13.4%、为9.6%。细胞总ERK的表达在各组间无明显差别。但不同浓度的“益肾蠲湿合剂”能显著地抑制血清所诱导的ERK1/2的磷酸化,最高抑制率达68%。结论:“益肾蠲湿合剂”可通过抑制ERK1/2的磷酸化来抑制GMC的增殖。
OBJECTIVE: To observe the effect of self-made compound prescription “Yishen Qushi mixture agent” on the proliferation of glomerular mesangial cells (GMC) and further explore its mechanism of action. METHODS: 1×10-3, 1×10-2, and 1×10-1 mg/mL of “Yishen Qushi Mixture” extract were applied to DMEM cultured GMC with a volume fraction of 10% serum, respectively, using MTT. Cell proliferation and phosphorylation of ERK1/2 were detected by Western Blot. RESULTS: 1×10-2 and 1×10-1mg/mL “Yishen Qushi Mixture” could significantly inhibit the proliferation of GMC. The inhibition rates were 13.4% and 9.6% at 12, 24, and 36 h, respectively. The total cell ERK expression was not significantly different among the groups. However, different concentrations of “Yishen Qushi Mixture ” can significantly inhibit serum-induced ERK1/2 phosphorylation, the highest inhibition rate of 68%. Conclusion: “Yishen Qushi Mixture” can inhibit the proliferation of GMC by inhibiting the phosphorylation of ERK1/2.