近年来杂交瘤技术的应用日趋广泛,在鉴定由杂交瘤细胞分泌的单克隆抗体的过程中,需要测定抗体 Ig 亚类。常规用琼脂双扩法进行测定,测定时需要较多的标准抗亚类血清。我们利用硝酸纤维膜作为固相载体,用 ELISA 法测定单克隆抗体 Ig 亚类,具有快速、简便、节省标准抗血清等优点。 In recent years, the application of hybridoma technology is becoming more and more widespread. In the process of identifying monoclonal antibodies secreted by hybridoma cells, it is necessary to determine the antibody Ig subclass. Conventional agar double expansion method for determination, the determination of the need for more standard anti-subclass serum. We use nitrocellulose membrane as the solid phase carrier, and measure the subclass of monoclonal antibody by ELISA, which has the advantages of fast, simple and save the standard antiserum.